Yang Beining, Li Wei, Mao Yuxuan, Zhao Yuanhui, Xue Yong, Xu Xinxing, Zhao Yilin, Liu Kang
Sanya Oceanographic Institution /College of Food Science and Engineering, Ocean University of China, Sanya/Qingdao, China.
State Key Laboratory of Marine Food Processing & Safety Control, Ocean University of China, Qingdao, Shandong, China.
Food Chem X. 2024 Feb 16;21:101236. doi: 10.1016/j.fochx.2024.101236. eCollection 2024 Mar 30.
Despite the favorable biocompatibility of natural antimicrobial peptides (AMPs), their scarcity limits their practical application. Through rational design, the activity of AMPs can be enhanced to expand their application. In this study, we selected a natural sturgeon epidermal mucus peptide, AP-16 (APATPAAPALLPLWLL), as the model molecule and studied its conformational regulation and antimicrobial activity through amino acid substitutions and -terminal lipidation. The structural and morphological transitions of the peptide self-assemblies were investigated using circular dichroism and transmission electron microscopy. Following amino acid substitution, the conformation of AL-16 (AKATKAAKALLKLWLL) did not change. Following -terminal alkylation, the C-AL-16 and C-AL-16 conformations changed from random coil to β-sheet or α-helix, and the self-assembly changed from nanofibers to nanospheres. AL-16, C-AL-16, and C-AL-16 presented significant antimicrobial activity against and at low concentrations. -terminal alkylation effectively extended the shelf life of . These results support the application of natural AMPs.
尽管天然抗菌肽（AMPs）具有良好的生物相容性，但其稀缺性限制了它们的实际应用。通过合理设计，可以增强AMPs的活性以扩大其应用范围。在本研究中，我们选择了一种天然鲟鱼表皮黏液肽AP-16（APATPAAPALLPLWLL）作为模型分子，并通过氨基酸替换和N端脂质化研究其构象调控和抗菌活性。使用圆二色光谱和透射电子显微镜研究了肽自组装体的结构和形态转变。氨基酸替换后，AL-16（AKATKAAKALLKLWLL）的构象未发生变化。N端烷基化后，C-AL-16和C-AL-16的构象从无规卷曲转变为β-折叠或α-螺旋，自组装体从纳米纤维转变为纳米球。AL-16、C-AL-16和C-AL-16在低浓度下对[具体菌种]和[具体菌种]均表现出显著的抗菌活性。N端烷基化有效地延长了[具体物质]的保质期。这些结果支持天然AMPs的应用。
