Department of Pharmacology and Toxicology, College of Pharmacy, University of Baghdad, Baghdad, Iraq.
J Med Life. 2023 Nov;16(11):1628-1632. doi: 10.25122/jml-2023-0082.
Ifosfamide (IFO), an alkylating chemotherapy agent, is known for its association with neurotoxicity and encephalopathy. This trial was designed to evaluate the protective action of daidzein (DZN) against IFO-induced neurotoxicity in male rats by determining the difference in certain inflammatory and apoptotic markers in the brain tissue of rats. Twenty-eight Wistar rats, weighing 120-150 g, were divided into four groups of seven rats: Group 1 (Control) received no treatment; Group 2 was orally administered DZN (100 mg/kg/day) for seven days; Group 3 received a single intraperitoneal (IP) dose of IFO (500 mg/kg); Group 4 received oral DZN (100 mg/kg/day) for one week prior to a single IP dose of IFO on the seventh day. Twenty-four hours post-treatment, serum and brain tissue samples were collected for analysis. The results indicated a significant increase in serum inflammatory markers (TNF-alpha, IL-6, and iNOS) and the anti-inflammatory marker (IL-10), along with elevated caspase-3 enzyme activity in the brain tissue of the IFO-treated group compared to the control group. Conversely, pre-treatment with DZN significantly reduced serum inflammatory markers and caspase-3 levels in tissue. The findings suggest that daidzein has anti-inflammatory and anti-apoptotic properties, potentially offering protection against IFO-induced neurotoxicity in rats.
异环磷酰胺（IFO）是一种烷化化疗药物，其与神经毒性和脑病有关。本试验旨在通过确定大鼠脑组织中某些炎症和凋亡标志物的差异，评估大豆甙元（DZN）对 IFO 诱导的神经毒性的保护作用。将 28 只体重为 120-150 克的 Wistar 大鼠分为四组，每组 7 只：第 1 组（对照组）未接受任何治疗；第 2 组连续 7 天口服 DZN（100mg/kg/天）；第 3 组单次腹腔内（IP）给予 IFO（500mg/kg）；第 4 组在第 7 天单次 IP 给予 IFO 前连续 7 天口服 DZN（100mg/kg/天）。治疗后 24 小时采集血清和脑组织样本进行分析。结果表明，与对照组相比，IFO 处理组大鼠血清炎症标志物（TNF-α、IL-6 和 iNOS）和抗炎标志物（IL-10）显著增加，脑组织中 caspase-3 酶活性升高。而 DZN 预处理显著降低了血清炎症标志物和组织中 caspase-3 的水平。研究结果表明，大豆甙元具有抗炎和抗凋亡作用，可能对 IFO 诱导的大鼠神经毒性具有保护作用。
