Uchida Y, Tomonaga M
Brain Res. 1985 Nov 25;348(1):100-6. doi: 10.1016/0006-8993(85)90364-6.
The effects of nerve growth factor (NGF) and heart-cell-conditioned medium (HCM) on the neurite regeneration of aged sympathetic neurons were investigated in culture. Investigation of HCM was carried out by two different methods: one was the use of whole HCM on collagen substratum, which reflected component(s) effective in solution (HCM-S); the other was the use of polyornithine (PORN)-binding component(s) (P-HCM). Superior cervical ganglion neurons prepared from male mice from 6 to 30 months of age were cultured in MEM-10% FCS on collagen or gelatin-PORN substratum for 3 days. The number of neurons with neurites and the length of neurites were quantified as neurite production and elongation, respectively. Neuronal survival was not affected by addition of NGF, HCM-S or P-HCM. Neurite production of early adult neurons was enhanced by NGF, HCM-S or P-HCM. In contrast, neurite production of aged neurons was enhanced by only HCM-S, but not NGF or P-HCM. HCM-S did not promote neurite elongation in neurons at any age. Neurite elongation of early adult neurons was enhanced by NGF or P-HCM. Neurite elongation of aged neurons was enhanced by P-HCM. However, responsiveness of NGF for neurite elongation varied according to substrata. No age-related difference was found in neurite production and elongation in the absence of NGF, HCM-S or P-HCM. These results indicate that responsiveness of aged sympathetic neurons is various in different growth factors.
在培养过程中研究了神经生长因子（NGF）和心脏细胞条件培养基（HCM）对老年交感神经元轴突再生的影响。通过两种不同方法对HCM进行了研究：一种是在胶原基质上使用全HCM，这反映了溶液中有效的成分（HCM-S）；另一种是使用聚鸟氨酸（PORN）结合成分（P-HCM）。从6至30月龄雄性小鼠制备的颈上神经节神经元在含10%胎牛血清的MEM培养基中，于胶原或明胶-PORN基质上培养3天。将有轴突的神经元数量和轴突长度分别量化为轴突产生和伸长。添加NGF、HCM-S或P-HCM不影响神经元存活。成年早期神经元的轴突产生可被NGF、HCM-S或P-HCM增强。相比之下，老年神经元的轴突产生仅被HCM-S增强，而不受NGF或P-HCM影响。HCM-S在任何年龄的神经元中均不促进轴突伸长。成年早期神经元的轴突伸长可被NGF或P-HCM增强。老年神经元的轴突伸长可被P-HCM增强。然而，NGF对轴突伸长的反应性因基质而异。在无NGF、HCM-S或P-HCM的情况下，未发现轴突产生和伸长存在年龄相关差异。这些结果表明，老年交感神经元对不同生长因子的反应性各不相同。
