Campenot R B
Section of Neurobiology and Behavior, Cornell University, Ithaca, NY 14853.
Brain Res. 1987 Dec 15;465(1-2):293-301. doi: 10.1016/0165-3806(87)90250-1.
Sprouting of neurites by sympathetic neurons from newborn rats was studied in compartmentalized cultures. The neuronal cell bodies resided in proximal compartments, and neurites penetrated silicone grease barriers and elongated within distal compartments. Nerve growth factor (NGF) was initially supplied at 1 microgram/ml in all compartments, but was subsequently withdrawn from proximal compartments and for a time was only supplied to distal neurites. Little or no neurite growth was observed in proximal compartments after NGF withdrawal, but reintroduction of NGF resulted in substantial neurite growth over the next few days which was shown to have originated from local sprouting within the proximal compartments. This result is distinct from previous work on NGF-enhanced nerve fiber elongation in demonstrating that quiescent, NGF-deprived regions of sympathetic neurons sprout neurites in response to local reexposure to NGF.
在分隔培养中研究了新生大鼠交感神经元的神经突萌发。神经元细胞体位于近端隔室，神经突穿透硅脂屏障并在远端隔室中伸长。神经生长因子（NGF）最初在所有隔室中以1微克/毫升的浓度供应，但随后从近端隔室中撤出，并且有一段时间仅供应给远端神经突。在撤出NGF后，近端隔室中几乎没有观察到神经突生长，但重新引入NGF导致在接下来的几天内神经突大量生长，这表明其起源于近端隔室内的局部萌发。这一结果与先前关于NGF增强神经纤维伸长的研究不同，表明交感神经元的静止、NGF剥夺区域会响应局部重新暴露于NGF而萌发出神经突。
