Guglielmi Marco Vito, Mastrodonato Maria, Semeraro Daniela, Mentino Donatella, Capriello Teresa, La Pietra Alessandra, Giarra Antonella, Scillitani Giovanni, Ferrandino Ida
Department of Biosciences, Biotechnologies and Environment, University of Bari Aldo Moro, Bari, Italy.
Department of Biology, University of Naples "Federico II", Naples, Italy.
Microsc Res Tech. 2024 Jul;87(7):1453-1466. doi: 10.1002/jemt.24529. Epub 2024 Feb 26.
Aluminum (Al) is used in everyday life and present in food drugs, packaging, industry, and agriculture. Although it is the most common metal in the Earth crust, a correlation has been demonstrated between its presence and various pathologies, even serious ones, especially of a neurological type. However, there is a histological gap regarding the role Al can have in contact with the covering and secreting epithelia. The alterations of the ventral and dorsal foot mucocytes and their secretions of the snail Eobania vermiculata caused by Al were investigated in situ by histochemical and lectin-histochemical techniques. Administration to different experimental groups took place for 3 and 9 days with 50 and 200 μM of AlCl. Several types of mucocytes were detected with a prevalent secretion of acid glycans in the foot of E. vermiculata. Sulfated glycans prevail in the dorsal region, with one type showing only fucosylated residues and another also having galactosaminylated and glycosaminylated residues. Carboxylated glycans prevail in the ventral region, with presence of galactosaminylated, glycosaminylated, and fucosylated residuals in both cells. Snails treated presented a general decrease of mucin amount in the secreting cells and affected the mucus composition. These changes could alter the rheological and functional properties of the mucus with possible implications for the health of the treated animals. RESEARCH HIGHLIGHTS: Snails were fed with Al-contaminated lettuce at different concentrations. In the foot mucocytes produced mucus with prevailing acidic glycans. In the treated resulted a reduction in the amount of mucus and an alteration of glycan composition.
铝（Al）在日常生活中广泛使用，存在于食品、药品、包装、工业和农业中。尽管它是地壳中最常见的金属，但已证实其存在与各种病理状况，甚至严重的病理状况，尤其是神经学类型的病理状况之间存在关联。然而，关于铝在与覆盖和分泌上皮接触时可能发挥的作用，存在组织学上的空白。通过组织化学和凝集素组织化学技术，对铝引起的蜗牛Eobania vermiculata腹侧和背侧足部粘液细胞及其分泌物的变化进行了原位研究。以50和200μM的AlCl对不同实验组进行给药，持续3天和9天。在E. vermiculata的足部检测到几种类型的粘液细胞，其中酸性聚糖分泌占主导。硫酸化聚糖在背侧区域占主导，一种类型仅显示岩藻糖基化残基，另一种还具有半乳糖胺基化和糖胺基化残基。羧化聚糖在腹侧区域占主导，两种细胞中均存在半乳糖胺基化、糖胺基化和岩藻糖基化残基。经处理的蜗牛分泌细胞中的粘蛋白量普遍减少，并影响了粘液成分。这些变化可能会改变粘液的流变学和功能特性，对受试动物的健康可能产生影响。研究亮点：给蜗牛喂食不同浓度的受铝污染生菜。足部粘液细胞产生的粘液中酸性聚糖占主导。处理后粘液量减少，聚糖组成发生改变。
