Chen Lixuan, Mo Qinglin, Wu Yingnan, Chen Wancheng, Deng Kaixian, Xiao Yang
Guangzhou University of Chinese Medicine, Guangzhou, China.
Jinshazhou Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
Free Radic Res. 2024 Feb;58(2):107-116. doi: 10.1080/10715762.2024.2320383. Epub 2024 Mar 8.
Oxidative stress injury is an important pathological factor of premature ovarian failure (POF). Salidroside, extracted from the Chinese herb-, has advantages in antioxidant characteristics. However, their therapeutic efficacy and mechanisms in POF have not been explored.
This study aims to assess the therapeutic effects of salidroside in chemotherapy-induced ovarian failure rats.
A POF rat model was established by injection of cyclophosphamide, followed by treatment with salidroside. The therapeutic effect of salidroside was evaluated based on hormone levels, follicle count, and reproductive ability. Oxidative stress injury was assessed by the detection of SOD enzyme activity and MDA levels. Differential gene expression of Keap1, Nrf2, HMOX1, NQO1, AMH, BMP15, and GDF9, were identified by qRT‑PCR. The protein expression of Keap1, Nrf2, P53, and Bcl-2 were detected by western blot.
Salidroside treatment markedly restored FSH, E2, and AMH hormone secretion levels, reduced follicular atresia, and increased antral follicle numbers in POF rats. In addition, salidroside improves fertility in POF rats, activates the Nrf2 signaling pathway, and reduces the level of oxidative stress. The recovery function of high dose salidroside (50 mg/kg) in a reproductive assay was significantly improved than that of lower dose salidroside (25 mg/kg). Meanwhile, the safety evaluation of salidroside treatment in rats showed that salidroside was safe for POF rats at doses of 25-50 mg/kg.
Salidroside therapy improved premature ovarian failure significantly through antioxidant function and activating Nrf2 signaling.
氧化应激损伤是卵巢早衰（POF）的重要病理因素。红景天苷是从中药中提取的，具有抗氧化特性。然而，其在POF中的治疗效果和机制尚未得到探索。
本研究旨在评估红景天苷对化疗诱导的卵巢功能衰竭大鼠的治疗效果。
通过注射环磷酰胺建立POF大鼠模型，然后用红景天苷进行治疗。基于激素水平、卵泡计数和生殖能力评估红景天苷的治疗效果。通过检测超氧化物歧化酶（SOD）酶活性和丙二醛（MDA）水平评估氧化应激损伤。通过qRT-PCR鉴定Keap1、Nrf2、血红素加氧酶1（HMOX1）、醌氧化还原酶1（NQO1）、抗苗勒管激素（AMH）、骨形态发生蛋白15（BMP15）和生长分化因子9（GDF9）的差异基因表达。通过蛋白质印迹法检测Keap1、Nrf2、P53和Bcl-2的蛋白表达。
红景天苷治疗显著恢复了POF大鼠的促卵泡生成素（FSH）、雌二醇（E2）和AMH激素分泌水平，减少了卵泡闭锁，并增加了窦卵泡数量。此外，红景天苷改善了POF大鼠的生育能力，激活了Nrf2信号通路，并降低了氧化应激水平。在生殖试验中，高剂量红景天苷（50mg/kg）的恢复功能明显优于低剂量红景天苷（25mg/kg）。同时，红景天苷治疗大鼠的安全性评估表明，25-50mg/kg剂量的红景天苷对POF大鼠是安全的。
红景天苷治疗通过抗氧化功能和激活Nrf2信号通路显著改善了卵巢早衰。
