State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, Zhejiang, China.
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, Zhejiang, China; Key Laboratory of Low-grade Energy Utilization Technologies and Systems of Ministry of Education, Chongqing University, Chongqing 400044, China; Dongtai Cibainian Bioengineering Company Limited, Yancheng 224200, China.
Bioresour Technol. 2024 Apr;397:130497. doi: 10.1016/j.biortech.2024.130497. Epub 2024 Feb 24.
To effectively improve biomass growth and flue-gas CO fixation of microalgae, acid-tolerant Euglena gracilis was modified with cobalt-60 γ-ray irradiation and polyethylene glycol (PEG) adaptive screening to obtain the mutant strain M800. The biomass dry weight and maximum CO fixation rate of M800 were both 1.47 times higher than that of wild strain, which was attributed to a substantial increase in key carbon fixation enzyme RuBisCO activity and photosynthetic pigment content. The high charge separation quantum efficiency in PSII reaction center, efficient light utilization and energy regulation that favors light conversion, were the underlying drivers of efficient photosynthetic carbon fixation in M800. M800 had stronger antioxidant capacity in sufficient high-carbon environment, alleviating lipid peroxidation damage. After adding 1 mM PEG, biomass dry weight of M800 reached 2.31 g/L, which was 79.1 % higher than that of wild strain. Cell proliferation of M800 was promoted, the apoptosis and necrosis rates decreased.
为了有效提高微藻的生物质生长和烟道气 CO 固定效率，采用钴-60γ射线辐照和聚乙二醇（PEG）适应性筛选的方法对耐酸的绿眼虫（Euglena gracilis）进行了修饰，获得了突变株 M800。M800 的生物质干重和最大 CO 固定率均比野生株高 1.47 倍，这归因于关键的碳固定酶 RuBisCO 活性和光合色素含量的大幅增加。在 PSII 反应中心具有高的电荷分离量子效率、有利于光转化的高效光能利用和能量调节，是 M800 高效光合作用碳固定的基础。在充足的高碳环境中，M800 具有更强的抗氧化能力，减轻了脂质过氧化损伤。添加 1mM PEG 后，M800 的生物质干重达到 2.31g/L，比野生株高 79.1%。M800 的细胞增殖得到促进，凋亡和坏死率降低。
