State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, Zhejiang, China.
Guangdong Energy Group Science and Technology Research Institute Co. Ltd., Guangzhou 510630, China.
Sci Total Environ. 2023 Apr 10;868:161629. doi: 10.1016/j.scitotenv.2023.161629. Epub 2023 Jan 16.
In order to improve CO biofixation efficiency of microalgae cultivated with coal-chemical flue gas, CO gradient domestication was employed to improve high-concentration CO tolerance and photoautotrophic growth of acid-tolerant Euglena gracilis. The dried biomass yield of photoautotrophic growth of E.gracilis increased from 1.09 g/L (wild-type strain) by 21 % to 1.32 g/L with CO gradient domestication to 15 % CO. The RuBisCO activity and biomass production of E.gracilis strain domesticated to 99 % CO were 2.63 and 3.4 times higher, respectively, than those of wild-type strain. The chlorophyll a and b contents were 2.52 and 1.79 times higher, respectively, than those of wild-type strain. Superoxide dismutase and catalase activities of 99 % CO-domesticated strain increased to 1.24 and 6 times, which reduced peroxide damage under high carbon stress and resulted in lower apoptotic and necrotic rates of domesticated strain. Thus, this work provides valuable guidance for CO fixation and adaptive evolution of E. gracilis in industrial flue gas.
为提高利用煤化工烟气培养的微藻 CO 固定效率，采用 CO 浓度梯度驯化法提高了耐酸嗜盐绿眼虫（Euglena gracilis）对高浓度 CO 的耐受性和光自养生长能力。经 CO 浓度梯度驯化至 15%CO 后，E. gracilis 光自养生长的干燥生物量产量从野生型菌株的 1.09 g/L 提高了 21%，达到 1.32 g/L。驯化至 99%CO 的 E. gracilis 菌株的 RuBisCO 活性和生物量产量分别比野生型菌株高 2.63 倍和 3.4 倍。叶绿素 a 和 b 的含量分别比野生型菌株高 2.52 倍和 1.79 倍。99%CO 驯化菌株的超氧化物歧化酶和过氧化氢酶活性分别增加到 1.24 倍和 6 倍，降低了高碳胁迫下的过氧化物损伤，导致驯化菌株的凋亡和坏死率降低。因此，这项工作为绿眼虫在工业烟气中的 CO 固定和适应性进化提供了有价值的指导。
