Institute of Nuclear Physics, Polish Academy of Sciences, Radzikowskiego 152, 31-342 Krakow, Poland; SOLARIS National Synchrotron Radiation Centre, Jagiellonian University, Czerwone Maki 98, 30-392 Krakow, Poland.
SOLARIS National Synchrotron Radiation Centre, Jagiellonian University, Czerwone Maki 98, 30-392 Krakow, Poland.
Biochim Biophys Acta Mol Cell Biol Lipids. 2024 May;1869(4):159468. doi: 10.1016/j.bbalip.2024.159468. Epub 2024 Feb 24.
Radiotherapy is one of the most commonly used cancer therapies with many benefits including low toxicity to healthy tissues. However, a major problem in radiotherapy is cancer radioresistance. To enhance the effect of this kind of therapy several approaches have been proposed such as the use of radiosensitizers. A combined treatment of radiotherapy and radiosensitizing drugs leads to a greater effect on cancer cells than anticipated from the addition of both responses (synergism). In this study, high-definition FT-IR imaging was applied to follow lipid accumulation in prostate cancer cells as a response to X-ray irradiation, radiosensitizing drugs, and a combined treatment of X-rays and the drugs. Lipid accumulation induced in the cells by an increasing X-ray dose and the presence of the drugs was analyzed using Principal Component Analysis and lipid staining. Finally, the synergistic effect of the combined therapy (X-rays and radiosensitizers) was confirmed by calculations of the integral intensity of the 2850 cm band.
放射治疗是最常用的癌症治疗方法之一，具有许多益处，包括对健康组织的低毒性。然而，放射治疗的一个主要问题是癌症放射抵抗性。为了增强这种治疗的效果，已经提出了几种方法，例如使用放射增敏剂。放射治疗和放射增敏药物的联合治疗对癌细胞的作用大于两种反应（协同作用）的预期相加。在这项研究中，高清晰度傅里叶变换红外成像被应用于跟踪前列腺癌细胞中脂质的积累，作为对 X 射线照射、放射增敏药物以及 X 射线和药物联合治疗的反应。使用主成分分析和脂质染色分析了细胞中由 X 射线剂量增加和药物存在引起的脂质积累。最后，通过计算 2850 cm 带的积分强度，证实了联合治疗（X 射线和放射增敏剂）的协同作用。
