The Molecular Medicine Key Laboratory of Liver Injury and Repair, College of Basic Medicine and Forensic Medicine, Henan University of Science and Technology, Luoyang, 471003, People's Republic of China.
Henan Center for Engineering and Technology Research On Prevention and Treatment of Liver Diseases, Luoyang, 471003, People's Republic of China.
J Physiol Sci. 2024 Feb 26;74(1):13. doi: 10.1186/s12576-024-00901-y.
The TGF-β1/Smad3-signaling pathway and gender differences were investigated in alcoholic liver fibrosis. Mice were divided into female normal, female model, male normal, and male model groups. Liver injury and fibrosis were assessed using histopathology and serology. Western blotting was performed to analyze the expression of relevant factors. HSC-T6 cells were divided into estradiol + saline, estradiol + ethanol, testosterone + saline, and testosterone + ethanol groups, and similar assessments were conducted in vitro. Compared with the female model group, the male model group exhibited significantly increased GPT, GOT, TNF-α, IL-6, and testosterone levels, fibrosis rate, and TGF-β1, Smad3, and PCNA expression, and significantly decreased estradiol levels and Caspase-3 expression. The apoptosis rate was higher in the estradiol + ethanol group than in the testosterone + ethanol group, although the testosterone + ethanol group exhibited significantly increased TNF-α, IL-6, Collagen-I, α-SMA, TGF-β1, Smad3, and PCNA expression, and significantly decreased Caspase-3 expression. Alcoholic liver fibrosis showed significant gender differences associated with the TGF-β1/Smad3-signaling pathway.
探讨了 TGF-β1/Smad3 信号通路与性别差异在酒精性肝纤维化中的作用。将小鼠分为雌性正常组、雌性模型组、雄性正常组和雄性模型组。通过组织病理学和血清学评估肝损伤和纤维化。采用 Western blot 分析相关因子的表达。将 HSC-T6 细胞分为雌二醇+生理盐水、雌二醇+乙醇、睾酮+生理盐水和睾酮+乙醇组，进行体外类似评估。与雌性模型组相比，雄性模型组的 GPT、GOT、TNF-α、IL-6 和睾酮水平、纤维化率以及 TGF-β1、Smad3 和 PCNA 表达显著升高，而雌二醇水平和 Caspase-3 表达显著降低。与睾酮+乙醇组相比，雌二醇+乙醇组的细胞凋亡率更高，尽管睾酮+乙醇组的 TNF-α、IL-6、Collagen-I、α-SMA、TGF-β1、Smad3 和 PCNA 表达显著升高，而 Caspase-3 表达显著降低。酒精性肝纤维化存在显著的性别差异，与 TGF-β1/Smad3 信号通路有关。
