Determination of 5-aminolevulinic acid in biological samples by high-performance liquid chromatography.
作者信息
Meisch H U, Reinle W, Wolf U
出版信息
Anal Biochem. 1985 Aug 15;149(1):29-34. doi: 10.1016/0003-2697(85)90473-7.
PMID:3840960
Abstract
5-Aminolevulinic acid (ALA), the common precursor of all naturally occurring tetrapyrroles, forms a stable condensation product with 2-amino-3-hydroxynaphthalene which can be identified by its fluorescence. Separation of the compound by reversed-phase high-performance liquid chromatography on RPC-18 columns allows its detection down to the picomolar range and can be successfully applied for ALA analysis in small biological samples. The reaction product of ALA with 2-amino-3-hydroxynaphthalene has been synthesized and characterized.
摘要
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