采用电子捕获检测气相色谱法测定生物体液中的δ-氨基乙酰丙酸。
Determination of delta-aminolaevulinic acid in biological fluids by gas-liquid chromatography with electron-capture detection.
作者信息
Gorchein A
出版信息
Biochem J. 1984 May 1;219(3):883-9. doi: 10.1042/bj2190883.
Abstract
A derivative of delta-aminolaevulinic acid (AmLev), 2-methyl-3-acetyl-4-(3-propionic acid pentafluorobenzyl ester)pyrrole, with favourable properties for g.l.c. with electron-capture detection, was synthesized. Less than 1 pg could be detected on the column. 6-Amino-5-oxohexanoic acid formed the analogous derivative under similar conditions and was used as the internal standard in the development of a highly sensitive and specific assay for AmLev. The method has been applied to peripheral-venous and umbilical-cord plasma and to cerebrospinal fluid of normal and porphyric subjects.
摘要
合成了δ-氨基乙酰丙酸（AmLev）的一种衍生物，即2-甲基-3-乙酰基-4-（3-丙酸五氟苄酯）吡咯，它具有利于采用电子捕获检测进行气相色谱分析的特性。在柱上可检测到的量少于1皮克。6-氨基-5-氧代己酸在类似条件下形成了类似的衍生物，并被用作开发一种针对AmLev的高灵敏度和特异性测定法的内标。该方法已应用于正常和卟啉症患者的外周静脉血和脐带血血浆以及脑脊液。
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本文引用的文献
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The occurrence and determination of delta-amino-levulinic acid and porphobilinogen in urine.尿中δ-氨基-γ-酮戊酸和胆色素原的出现及测定
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The purification and properties of delta-aminolaevulic acid dehydrase.δ-氨基乙酰丙酸脱水酶的纯化及性质
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Porphobilinogen.胆色素原
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Heme biosynthesis in intermittent acute prophyria: decreased hepatic conversion of porphobilinogen to porphyrins and increased delta aminolevulinic acid synthetase activity.间歇性急性卟啉病中的血红素生物合成：肝内胆色素原向卟啉的转化减少，δ-氨基-γ-酮戊酸合成酶活性增加。
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A gas chromatographic method for the determination of aminoketones in urine and serum.一种用于测定尿液和血清中氨基酮的气相色谱法。
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