LaFlamme S E, Kramer F R, Mills D R
Nucleic Acids Res. 1985 Dec 9;13(23):8425-40. doi: 10.1093/nar/13.23.8425.
Pausing during the transcription of MDV-1 cDNA by Escherichia coli RNA polymerase was compared with pausing during the replication of MDV-1 RNA by Q beta replicase. MDV-1 RNA is able to form many strong hairpin structures, and Q beta replicase pauses after the synthesis of each [Mills et al. (1978) Cell 15, 541-550]. Although the transcripts were virtually identical to MDV-1 RNA, the locations at which RNA polymerase paused were different and apparently were not related to sequences that can form hairpins. These results indicate that hairpin stability, per se, cannot be used to predict the occurrence of pausing during transcription. Four pauses that occur within a 5-nucleotide region were studied in detail. Insertions and deletions were made in the template DNA to determine the contribution made by the surrounding sequences to these pauses. The results indicate that some of the pauses require the presence of particular upstream sequences, while others are unaffected by the template modifications. Thus, there are at least two different transcriptional pausing mechanisms: one depends on the nature of upstream sequences, while the other is independent of upstream sequences.
将大肠杆菌RNA聚合酶转录MDV - 1 cDNA过程中的暂停与Qβ复制酶复制MDV - 1 RNA过程中的暂停进行了比较。MDV - 1 RNA能够形成许多强大的发夹结构，并且Qβ复制酶在每次合成后都会暂停[米尔斯等人（1978年），《细胞》15卷，541 - 550页]。尽管转录本与MDV - 1 RNA几乎相同，但RNA聚合酶暂停的位置不同，且显然与能够形成发夹的序列无关。这些结果表明，发夹稳定性本身不能用于预测转录过程中暂停的发生。对在一个5核苷酸区域内发生的四个暂停进行了详细研究。在模板DNA中进行插入和缺失操作，以确定周围序列对这些暂停的影响。结果表明，一些暂停需要特定上游序列的存在，而其他暂停不受模板修饰的影响。因此，至少有两种不同的转录暂停机制：一种取决于上游序列的性质，而另一种与上游序列无关。
