Nishihara T, Mills D R, Kramer F R
J Biochem. 1983 Mar;93(3):669-74. doi: 10.1093/jb/93.3.669.
Fragments of MDV-1 RNA (a small, naturally occurring template for Q beta replicase) that were missing nucleotides at either their 5' end or their 3' end were still able to form a complex with Q beta replicase. By assaying the binding ability of fragments of different length, it was established that the binding site for Q beta replicase is determined by nucleotide sequences that are located near the middle of MDV-1 RNA. Fragments missing nucleotides at their 5' end were able to serve as templates for the synthesis of complementary strands, but fragments missing nucleotides at their 3' end were inactive, indicating that the 3'-terminal region of the template is required for the initiation of RNA synthesis. The nucleotide sequences of both the 3' terminus and the central binding region of MDV-1 (+) RNA are almost identical to sequences at the 3' terminus and at an internal region of Q beta (-) RNA.
MDV - 1 RNA（一种用于Qβ复制酶的小的天然存在模板）在其5'端或3'端缺失核苷酸的片段仍能够与Qβ复制酶形成复合物。通过检测不同长度片段的结合能力，确定了Qβ复制酶的结合位点由位于MDV - 1 RNA中间附近的核苷酸序列决定。在其5'端缺失核苷酸的片段能够作为合成互补链的模板，但在其3'端缺失核苷酸的片段无活性，这表明模板的3'末端区域是RNA合成起始所必需的。MDV - 1（+）RNA的3'末端和中央结合区域的核苷酸序列与Qβ（-）RNA的3'末端和内部区域的序列几乎相同。
