Benes L, Velcovský V, Rotreklová E
Gen Physiol Biophys. 1985 Dec;4(6):631-9.
3H-AMD binding to DNA in interphase nuclei was tested on asynchronous and synchronous LS/BL cell populations under physiological conditions and after exposure to gamma rays (60Co). 3H-AMD binding to DNA in an asynchronous cell population appeared to be nearly constant and independent of 3H-AMD concentration. However, in comparing individual cells, a great variability could be observed. In synchronized cells the DNA accessibility for 3H-AMD binding changed in the course of the cell cycle, with a maximum occurring at the late G1-phase (13.95 X 10(-12) mumol/nucleus) and a minimum at the late G2-phase (2.63 X 10(-12) mumol/nucleus). In irradiated cells the DNA capacity for 3H-AMD binding was growing with the increasing dose (5-80 Gy) from 4.9 to 11.2 X 10(-12) mumol 3H-AMD/nucleus.
在生理条件下以及暴露于γ射线（60Co）后，对异步和同步的LS/BL细胞群体测试了3H - AMD与间期细胞核中DNA的结合情况。在异步细胞群体中，3H - AMD与DNA的结合似乎几乎恒定，且与3H - AMD浓度无关。然而，在比较单个细胞时，可以观察到很大的变异性。在同步细胞中，3H - AMD结合的DNA可及性在细胞周期过程中发生变化，在G1期后期达到最大值（13.95×10(-12) μmol/细胞核），在G2期后期达到最小值（2.63×10(-12) μmol/细胞核）。在受辐照的细胞中，3H - AMD结合的DNA能力随着剂量（5 - 80 Gy）的增加而从4.9增长到11.2×10(-12) μmol 3H - AMD/细胞核。
