Niiazmatov A A, Kushch A A, Zelenin A V
Ontogenez. 1978;9(4):355-62.
The response of lymph node lymphocytes stimulated to proliferation at different stages of maturation by cell seeding at the high cell density was studied. The binding of acridine orange (A3) and 3H-actinomycin D (3H-AMD) to the cell nuclei was used as a criterion of the functional state of lymphocyte chromatin. The "crowding" stimulation of the lymph node lumphocytes resulted in the increase of AO and 3H-AMD binding by the cells of different sizes, i.e. at different stages of maturation. The degree of binding differs in cells of different size and the increase is expressed to a greater extent in small lymphocytes. The cell treatment with 0.2--0.3M sodium chloride solutions eliminates the differences in AO and 3H-AMD binding between the activated an non-activated lymphocytes of all sizes what appears to be due to the removal of some weakly bound non-histone proteins from the activated cells. The differences in AO and 3H-AMD binding between the cells of different sizes are retained after this treatment. Hence, the proteins removed from the chromatin under the effect of such a treatment are not responsible for the chromatin changes accompanying the cell maturation.
研究了通过高细胞密度接种细胞，在不同成熟阶段被刺激增殖的淋巴结淋巴细胞的反应。吖啶橙（A3）和3H-放线菌素D（3H-AMD）与细胞核的结合被用作淋巴细胞染色质功能状态的标准。淋巴结淋巴细胞的“拥挤”刺激导致不同大小的细胞（即不同成熟阶段的细胞）对AO和3H-AMD的结合增加。不同大小细胞的结合程度不同，且在小淋巴细胞中增加程度更大。用0.2 - 0.3M氯化钠溶液处理细胞消除了所有大小的活化淋巴细胞和未活化淋巴细胞之间AO和3H-AMD结合的差异，这似乎是由于从活化细胞中去除了一些弱结合的非组蛋白。这种处理后，不同大小细胞之间AO和3H-AMD结合的差异仍然存在。因此，在这种处理作用下从染色质中去除的蛋白质与伴随细胞成熟的染色质变化无关。
