• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于改良痘苗病毒安卡拉（MVA）的疫苗可保护食蟹猕猴免受东部马脑炎病毒气溶胶攻击致死。

MVA-based vaccines are protective against lethal eastern equine encephalitis virus aerosol challenge in cynomolgus macaques.

作者信息

Beddingfield Brandon J, Plante Kenneth S, Plante Jessica A, Weaver Scott C, Bose Sarah, Krzykwa Clara, Chirichella Nicole, Redmann Rachel K, Seiler Stephanie Z, Dufour Jason, Blair Robert V, Endt Kathrin, Volkmann Ariane, Maness Nicholas J, Roy Chad J

机构信息

Division of Microbiology, Tulane National Primate Research Center, Covington, LA, USA.

Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA.

出版信息

NPJ Vaccines. 2024 Feb 27;9(1):47. doi: 10.1038/s41541-024-00842-y.

DOI:10.1038/s41541-024-00842-y
PMID:38413593
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10899228/
Abstract

MVA-based monovalent eastern equine encephalitis virus (MVA-BN-EEEV) and multivalent western, eastern, and Venezuelan equine encephalitis virus (MVA-BN-WEV) vaccines were evaluated in the cynomolgus macaque aerosol model of EEEV infection. Macaques vaccinated with two doses of 5 × 10 infectious units of the MVA-BN-EEEV or MVA-BN-WEV vaccine by the intramuscular route rapidly developed robust levels of neutralizing antibodies to EEEV that persisted at high levels until challenge at day 84 via small particle aerosol delivery with a target inhaled dose of 10 PFU of EEEV FL93-939. Robust protection was observed, with 7/8 animals receiving MVA-BN-EEEV and 100% (8/8) animals receiving MVA-BN-WEV surviving while only 2/8 mock vaccinated controls survived lethal challenge. Complete protection from viremia was afforded by both vaccines, with near complete protection from vRNA loads in tissues and any pathologic evidence of central nervous system damage. Overall, the results indicate both vaccines are effective in eliciting an immune response that is consistent with protection from aerosolized EEEV-induced disease.

摘要

在食蟹猴气溶胶感染东部马脑炎病毒（EEEV）模型中评估了基于改良痘苗病毒安卡拉（MVA）的单价东部马脑炎病毒疫苗（MVA-BN-EEEV）和多价西部、东部及委内瑞拉马脑炎病毒疫苗（MVA-BN-WEV）。通过肌肉注射途径用两剂5×10感染单位的MVA-BN-EEEV或MVA-BN-WEV疫苗接种的食蟹猴，迅速产生了针对EEEV的高效中和抗体水平，这些抗体水平在第84天通过小颗粒气溶胶递送、目标吸入剂量为10 PFU的EEEV FL93-939进行攻击之前一直保持在高水平。观察到了强大的保护作用，接受MVA-BN-EEEV的8只动物中有7只存活，接受MVA-BN-WEV的动物100%（8/8）存活，而仅接种安慰剂的对照组8只动物中只有2只在致死性攻击中存活。两种疫苗都提供了完全的病毒血症保护，对组织中的病毒RNA载量以及中枢神经系统损伤的任何病理证据提供了近乎完全的保护。总体而言，结果表明两种疫苗在引发免疫反应方面都是有效的，这种免疫反应与免受气溶胶化EEEV诱导疾病的保护作用一致。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a1b/10899228/07eabbe1979f/41541_2024_842_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a1b/10899228/b774e87262b6/41541_2024_842_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a1b/10899228/dd61b6e83944/41541_2024_842_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a1b/10899228/569545fd445a/41541_2024_842_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a1b/10899228/d07996a56659/41541_2024_842_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a1b/10899228/07eabbe1979f/41541_2024_842_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a1b/10899228/b774e87262b6/41541_2024_842_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a1b/10899228/dd61b6e83944/41541_2024_842_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a1b/10899228/569545fd445a/41541_2024_842_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a1b/10899228/d07996a56659/41541_2024_842_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a1b/10899228/07eabbe1979f/41541_2024_842_Fig5_HTML.jpg

相似文献

1
MVA-based vaccines are protective against lethal eastern equine encephalitis virus aerosol challenge in cynomolgus macaques.基于改良痘苗病毒安卡拉（MVA）的疫苗可保护食蟹猕猴免受东部马脑炎病毒气溶胶攻击致死。
NPJ Vaccines. 2024 Feb 27;9(1):47. doi: 10.1038/s41541-024-00842-y.
2
A Monovalent and Trivalent MVA-Based Vaccine Completely Protects Mice Against Lethal Venezuelan, Western, and Eastern Equine Encephalitis Virus Aerosol Challenge.单价和三价 MVA 疫苗可完全保护小鼠免受致命委内瑞拉、西部和东部马脑炎病毒气溶胶挑战。
Front Immunol. 2021 Jan 19;11:598847. doi: 10.3389/fimmu.2020.598847. eCollection 2020.
3
Protective efficacy of monovalent and trivalent recombinant MVA-based vaccines against three encephalitic alphaviruses.单价和三价基于重组 MVA 的疫苗对三种脑炎甲病毒的保护效力。
Vaccine. 2018 Aug 16;36(34):5194-5203. doi: 10.1016/j.vaccine.2018.06.064. Epub 2018 Jul 20.
4
Safety and immunogenicity of a novel trivalent recombinant MVA-based equine encephalitis virus vaccine: A Phase 1 clinical trial.新型三价重组 MVA 基于马脑炎病毒疫苗的安全性和免疫原性：一项 I 期临床试验。
Vaccine. 2024 Apr 11;42(10):2695-2706. doi: 10.1016/j.vaccine.2024.03.011. Epub 2024 Mar 16.
5
Combined alphavirus replicon particle vaccine induces durable and cross-protective immune responses against equine encephalitis viruses.组合α病毒复制子颗粒疫苗可诱导针对马脑炎病毒的持久且具有交叉保护作用的免疫反应。
J Virol. 2014 Oct;88(20):12077-86. doi: 10.1128/JVI.01406-14. Epub 2014 Aug 13.
6
A chimeric Sindbis-based vaccine protects cynomolgus macaques against a lethal aerosol challenge of eastern equine encephalitis virus.基于嵌合辛德比斯病毒的疫苗可预防食蟹猕猴免受东部马脑炎病毒致死性气溶胶攻击。
Vaccine. 2013 Mar 1;31(11):1464-70. doi: 10.1016/j.vaccine.2013.01.014. Epub 2013 Jan 16.
7
Comparative pathology study of Venezuelan, eastern, and western equine encephalitis viruses in non-human primates.在非人类灵长类动物中比较委内瑞拉、东方和西方马脑炎病毒的病理学研究。
Antiviral Res. 2020 Oct;182:104875. doi: 10.1016/j.antiviral.2020.104875. Epub 2020 Aug 2.
8
A Multiagent Alphavirus DNA Vaccine Delivered by Intramuscular Electroporation Elicits Robust and Durable Virus-Specific Immune Responses in Mice and Rabbits and Completely Protects Mice against Lethal Venezuelan, Western, and Eastern Equine Encephalitis Virus Aerosol Challenges.肌肉内电穿孔传递的多价丙型肝炎病毒 DNA 疫苗在小鼠和兔中引发强大且持久的病毒特异性免疫应答，并完全保护小鼠免受致死性委内瑞拉、西部和东部马脑炎病毒气溶胶挑战。
J Immunol Res. 2018 Jun 3;2018:8521060. doi: 10.1155/2018/8521060. eCollection 2018.
9
Recombinant Isfahan Virus and Vesicular Stomatitis Virus Vaccine Vectors Provide Durable, Multivalent, Single-Dose Protection against Lethal Alphavirus Challenge.重组伊斯法罕病毒和水疱性口炎病毒疫苗载体可提供针对致死性甲病毒攻击的持久、多价、单剂量保护。
J Virol. 2017 Mar 29;91(8). doi: 10.1128/JVI.01729-16. Print 2017 Apr 15.
10
Novel Insect-Specific Eilat Virus-Based Chimeric Vaccine Candidates Provide Durable, Mono- and Multivalent, Single-Dose Protection against Lethal Alphavirus Challenge.基于新型昆虫特异性埃拉特病毒的嵌合疫苗候选物可提供持久的单剂量和多价保护，抵御致死性甲病毒攻击。
J Virol. 2018 Jan 30;92(4). doi: 10.1128/JVI.01274-17. Print 2018 Feb 15.

引用本文的文献

1
Eastern Equine Encephalitis Virus: The Importance of Metabolism and Aging.东部马脑炎病毒：新陈代谢与衰老的重要性
Int J Mol Sci. 2024 Dec 12;25(24):13318. doi: 10.3390/ijms252413318.
2
Syrian Hamsters Model Does Not Reflect Human-like Disease after Aerosol Exposure to Encephalitic Alphaviruses.叙利亚仓鼠模型在气溶胶暴露于脑炎甲病毒后不能反映类人疾病。
Methods Protoc. 2024 May 15;7(3):42. doi: 10.3390/mps7030042.

本文引用的文献

1
Reduced Respiratory Syncytial Virus Load, Symptoms, and Infections: A Human Challenge Trial of MVA-BN-RSV Vaccine.呼吸道合胞病毒载量、症状和感染减少：MVA-BN-RSV 疫苗人体挑战试验。
J Infect Dis. 2023 Oct 18;228(8):999-1011. doi: 10.1093/infdis/jiad108.
2
First-in-human study to evaluate safety, tolerability, and immunogenicity of heterologous regimens using the multivalent filovirus vaccines Ad26.Filo and MVA-BN-Filo administered in different sequences and schedules: A randomized, controlled study.人体首次研究评估使用不同顺序和方案给药的多价丝状病毒疫苗 Ad26.Filo 和 MVA-BN-Filo 的异源方案的安全性、耐受性和免疫原性：一项随机、对照研究。
PLoS One. 2022 Oct 5;17(10):e0274906. doi: 10.1371/journal.pone.0274906. eCollection 2022.
3
A Monovalent and Trivalent MVA-Based Vaccine Completely Protects Mice Against Lethal Venezuelan, Western, and Eastern Equine Encephalitis Virus Aerosol Challenge.单价和三价 MVA 疫苗可完全保护小鼠免受致命委内瑞拉、西部和东部马脑炎病毒气溶胶挑战。
Front Immunol. 2021 Jan 19;11:598847. doi: 10.3389/fimmu.2020.598847. eCollection 2020.
4
Physiological and immunological changes in the brain associated with lethal eastern equine encephalitis virus in macaques.与致命性东部马脑炎病毒在猕猴中相关的大脑的生理和免疫变化。
PLoS Pathog. 2021 Feb 3;17(2):e1009308. doi: 10.1371/journal.ppat.1009308. eCollection 2021 Feb.
5
Climate change and viral emergence: evidence from Aedes-borne arboviruses.气候变化与病毒出现：来自伊蚊传播虫媒病毒的证据
Curr Opin Virol. 2020 Feb;40:41-47. doi: 10.1016/j.coviro.2020.05.001. Epub 2020 Jun 20.
6
Notes from the Field: Multistate Outbreak of Eastern Equine Encephalitis Virus - United States, 2019.实地记录：2019年美国东部马脑炎病毒多州暴发
MMWR Morb Mortal Wkly Rep. 2020 Jan 17;69(2):50-51. doi: 10.15585/mmwr.mm6902a4.
7
Eastern Equine Encephalitis Virus - Another Emergent Arbovirus in the United States.东部马脑炎病毒——美国另一种新出现的虫媒病毒。
N Engl J Med. 2019 Nov 21;381(21):1989-1992. doi: 10.1056/NEJMp1914328.
8
Phase 3 Efficacy Trial of Modified Vaccinia Ankara as a Vaccine against Smallpox.改良安卡拉牛痘疫苗作为天花疫苗的 3 期疗效试验。
N Engl J Med. 2019 Nov 14;381(20):1897-1908. doi: 10.1056/NEJMoa1817307.
9
Modified Vaccinia Virus Ankara Can Induce Optimal CD8 T Cell Responses to Directly Primed Antigens Depending on Vaccine Design.改良安卡拉痘苗病毒可根据疫苗设计诱导直接刺激抗原的最佳 CD8 T 细胞应答。
J Virol. 2019 Oct 15;93(21). doi: 10.1128/JVI.01154-19. Print 2019 Nov 1.
10
A virus-like particle vaccine prevents equine encephalitis virus infection in nonhuman primates.一种类似病毒的颗粒疫苗可预防非人灵长类动物感染脑炎病毒。
Sci Transl Med. 2019 May 15;11(492). doi: 10.1126/scitranslmed.aav3113.