Kanaya E, Yanagawa H
Nucleic Acids Symp Ser. 1985(16):181-4.
Cyanogen bromide was found to be a highly effective reagent for the coupling of oligoadenylates on a polyuridylic acid template in aqueous solution. Imidazole and divalent metal ions were required for the reaction. Hexaadenylic acid was converted to (pA)12, (pA)18, (pA)24, (pA)30 and (pA)36 in the presence of 0.01 M Co2+ and imidazole. When the reaction was carried out at 25 degrees C for 3 days, the oligomers were obtained in a 34% overall yield.
发现溴化氰是一种在水溶液中于聚尿苷酸模板上偶联寡聚腺苷酸的高效试剂。该反应需要咪唑和二价金属离子。在0.01 M Co2+和咪唑存在的情况下，六聚腺苷酸被转化为(pA)12、(pA)18、(pA)24、(pA)30和(pA)36。当反应在25℃下进行3天时，寡聚物的总产率为34%。
