Template-directed synthesis of oligoadenylate. Template effect of oligouridylates and catalytic activity of Pb2+ ion.

Template-directed condensation of adenosine-5'-phosphorimidazolide was conducted in the presence of oligouridylate template. Oligouridylates with more than eight chain units can serve as a template and facilitate oligoadenylate formation. Internucleotide linkage of the oligoadenylate obtained by this reaction was mainly 2'-5'. Addition of Pb2+ ion catalyst to this reaction system promoted the formation of long oligoadenylates. The template with Pb2+ ion catalyst preferentially gave 3'-5' linked oligoadenylates.