Sawai H
Nucleic Acids Symp Ser. 1980(8):s77-9.
Template-directed condensation of adenosine-5'-phosphorimidazolide was conducted in the presence of oligouridylate template. Oligouridylates with more than eight chain units can serve as a template and facilitate oligoadenylate formation. Internucleotide linkage of the oligoadenylate obtained by this reaction was mainly 2'-5'. Addition of Pb2+ ion catalyst to this reaction system promoted the formation of long oligoadenylates. The template with Pb2+ ion catalyst preferentially gave 3'-5' linked oligoadenylates.
在寡尿苷酸模板存在的情况下，进行了腺苷-5'-磷酰咪唑化物的模板导向缩合反应。具有超过八个链单元的寡尿苷酸可以作为模板并促进寡腺苷酸的形成。通过该反应获得的寡腺苷酸的核苷酸间连接主要是2'-5'。向该反应体系中添加Pb2+离子催化剂促进了长链寡腺苷酸的形成。含有Pb2+离子催化剂的模板优先生成3'-5'连接的寡腺苷酸。
