Molecular Mycology Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore,560064, India.
Computational Biology, The Institute of Mathematical Sciences, Chennai, 600113, India.
Med Mycol. 2024 Mar 7;62(3). doi: 10.1093/mmy/myae018.
Candida auris poses threats to the global medical community due to its multidrug resistance, ability to cause nosocomial outbreaks and resistance to common sterilization agents. Different variants that emerged at different geographical zones were classified as clades. Clade-typing becomes necessary to track its spread, possible emergence of new clades, and to predict the properties that exhibit a clade bias. We previously reported a colony-Polymerase Chain Reaction-based, clade-identification method employing whole genome alignments and identification of clade-specific sequences of four major geographical clades. Here, we expand the panel by identifying clade 5 which was later isolated in Iran, using specific primers designed through in silico analyses.
由于其多药耐药性、引起医院感染爆发的能力以及对常见灭菌剂的耐药性,耳念珠菌对全球医疗界构成威胁。在不同地理区域出现的不同变体被归类为进化枝。对其进行进化枝分型对于追踪其传播、可能出现新的进化枝以及预测表现出进化枝偏向的特性是必要的。我们之前报道了一种基于菌落聚合酶链反应的进化枝鉴定方法,该方法采用全基因组比对和鉴定四个主要地理进化枝的进化枝特异性序列。在这里,我们通过使用通过计算机分析设计的特定引物,鉴定了后来在伊朗分离出的进化枝 5,从而扩展了该小组。