Peterson Shelley, Daigle Jade, Dueck Codey, Nagasawa Audra, Mulvey Michael, Mangat Chand S
Wastewater Surveillance Unit, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB.
Centre for Population Health Data, Statistics Canada, Ottawa, ON.
Can Commun Dis Rep. 2023 May 1;49(5):213-220. doi: 10.14745/ccdr.v49i05a07.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern are associated with increased infectivity, severity, and mortality of coronavirus disease 2019 (COVID-19) and have been increasingly detected in clinical and wastewater surveillance in Canada and internationally. In this study, we present a real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay for detection of the N gene D377Y mutation associated with the SARS-CoV-2 Delta variant in wastewater.
Wastewater samples (n=980) were collected from six cities and 17 rural communities across Canada from July to November 2021 and screened for the D377Y mutation.
The Delta variant was detected in all major Canadian cities and northern remote regions, and half of the southern rural communities. The sensitivity and specificity of this assay were sufficient for detection and quantitation of the Delta variant in wastewater to aid in rapid population-level screening and surveillance.
This study demonstrates a novel cost-effective RT-qPCR assay for tracking the spread of the SARS-CoV-2 Delta variant. This rapid assay can be easily integrated into current wastewater surveillance programs to aid in population-level variant tracking.
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的关注变异株与2019冠状病毒病（COVID-19）的传染性、严重程度和死亡率增加有关，并且在加拿大和国际上的临床和废水监测中越来越多地被检测到。在本研究中，我们提出了一种实时定量逆转录聚合酶链反应（RT-qPCR）检测方法，用于检测与废水中SARS-CoV-2 Delta变异株相关的N基因D377Y突变。
2021年7月至11月从加拿大六个城市和17个农村社区收集了废水样本（n = 980），并对D377Y突变进行筛查。
在加拿大所有主要城市和北部偏远地区以及一半的南部农村社区检测到了Delta变异株。该检测方法的灵敏度和特异性足以检测和定量废水中的Delta变异株，有助于快速进行人群水平的筛查和监测。
本研究展示了一种用于追踪SARS-CoV-2 Delta变异株传播的新型经济高效的RT-qPCR检测方法。这种快速检测方法可以轻松整合到当前的废水监测计划中，以协助进行人群水平的变异株追踪。
