Nayak Anjan Kumar, Golive Prasanthi, Sasmal Arundhati, Devanna B N, Anilkumar C, Mukherjee Arup Kumar, Dash Soumya Shephalika, Das Mohapatra Shyamaranjan, Subudhi Hatanath
Department of Entomology, College of Agriculture, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha 751003 India.
Crop Protection Division, ICAR-National Rice Research Institute, Cuttack, Odisha 753006 India.
3 Biotech. 2024 Mar;14(3):90. doi: 10.1007/s13205-024-03930-x. Epub 2024 Feb 25.
Rice production faces a significant threat from the rice leaffolder, . To address this challenge, growing resistant varieties stands out as a sustainable and eco-friendly pest management strategy. This necessitates identifying resistant sources and understanding their inheritance patterns through employing DNA markers for marker-assisted resistance breeding. Our study involves screening for resistant cultivars following the SES of IRRI, assessing genetic diversity among landraces using molecular markers, and identifying genomic regions associated with resistance. Screening indicated that 33.33%, 27.08%, 19.79%, and 19.80% of genotypes were resistant, moderately resistant, susceptible, and admixture, respectively. Landraces were categorized into three clusters, with clusters I and II predominantly containing moderately resistant and resistant cultivars, and cluster III mainly susceptible types. Molecular variance analysis revealed 12% variation among populations and 88% within the population. Simple linear regression identified significant marker-trait associations, with markers RM 162 and RM 284 on chromosomes 6 and 8, respectively, found highly associated with leaffolder resistance. Phenotypic variation in leaffolder damage correlated highly with the allelic effects of these markers. Further confirmation of marker linkage with resistance loci was established through independent assays on highly resistant and susceptible genotypes. The information derived from genetic diversity and marker-trait associations will be useful for future marker-assisted resistance breeding programs, enhancing the sustainability of rice production.
水稻生产面临着来自稻纵卷叶螟的重大威胁。为应对这一挑战，种植抗性品种成为一种可持续且环保的害虫管理策略。这就需要通过使用DNA标记进行标记辅助抗性育种来鉴定抗性来源并了解其遗传模式。我们的研究包括按照国际水稻研究所的标准评价系统筛选抗性品种，利用分子标记评估地方品种间的遗传多样性，以及鉴定与抗性相关的基因组区域。筛选结果表明，分别有33.33%、27.08%、19.79%和19.80%的基因型为抗性、中抗、感病和混合类型。地方品种被分为三个类群，其中类群I和II主要包含中抗和抗性品种，类群III主要是感病类型。分子方差分析显示群体间变异为12%，群体内变异为88%。简单线性回归确定了显著的标记-性状关联，分别位于第6和第8染色体上的标记RM 162和RM 284与稻纵卷叶螟抗性高度相关。稻纵卷叶螟危害的表型变异与这些标记的等位基因效应高度相关。通过对高抗和感病基因型的独立分析进一步证实了标记与抗性位点的连锁关系。从遗传多样性和标记-性状关联中获得的信息将有助于未来的标记辅助抗性育种计划，提高水稻生产的可持续性。
