Shirota Yuma, Otani Taketo, Wasada Sayo, Ito Shunsuke, Mieda Tokue, Nakamura Kazuhiro
Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences, 3-39-22, Showa-machi, Maebashi, Gunma 371-8511, Japan.
Department of Orthopedic Surgery, Gunma University Graduate School of Medicine, 3-39-22, Showa-machi, Maebashi, Gunma 371-8511, Japan.
IBRO Neurosci Rep. 2024 Feb 16;16:345-352. doi: 10.1016/j.ibneur.2024.02.005. eCollection 2024 Jun.
Spinal cord injury (SCI) is a devastating mechanical trauma. Although locomotion of model animals that mimic contusion SCI was actively examined, locomotion after penetrating SCI caused by sharp objects was not extensively studied. Severity of walking difficulty after partial transection of the spinal cord including penetrating SCI likely depends on the regions affected. Therefore, we compared beam walking and overground walking between mice after penetrating SCI at inner spinal cord region and mice with the injury at the outer region. Mice with the both penetrating SCIs did not display changes in beam walking. When appearance and movements of hindlimbs during overground walking was rated using Basso Mouse Scale for locomotion (BMS), however, mice with inner penetrating SCI showed low score shortly after the SCI. However, the score became high at later time points, as seen in contusion SCI mice. By contrast, BMS score did not decrease shortly after the outer penetrating SCI. However, the score became low 3 weeks after the SCI. As quantitative values during overground walking, movement duration in an open field were shorter at 1 day after the two penetrating SCIs. However, slower moving speed and fewer number of movement at 1 day were specific to mice with inner and outer penetrating SCIs, respectively. Moreover, BMS score was correlated with walking distance in open field only in mice with inner penetrating SCI. Thus, inner and outer penetrating SCI cause difficulty in overground walking with different severity and progress.
脊髓损伤（SCI）是一种毁灭性的机械创伤。尽管对模拟挫伤性SCI的模型动物的运动进行了积极研究，但由尖锐物体导致的穿透性SCI后的运动情况尚未得到广泛研究。包括穿透性SCI在内的脊髓部分横断后步行困难的严重程度可能取决于受影响的区域。因此，我们比较了脊髓内部区域发生穿透性SCI的小鼠与脊髓外部区域受伤的小鼠在梁式行走和地面行走方面的差异。两种穿透性SCI的小鼠在梁式行走方面均未表现出变化。然而，当使用巴索小鼠运动量表（BMS）对地面行走时后肢的外观和运动进行评分时，脊髓内部发生穿透性SCI的小鼠在SCI后不久评分较低。不过，与挫伤性SCI小鼠一样，该评分在后期时间点升高。相比之下，脊髓外部发生穿透性SCI后不久BMS评分并未降低。然而，SCI后3周评分降低。作为地面行走期间的定量值，两种穿透性SCI后1天在开放场地中的运动持续时间均较短。然而，1天时移动速度较慢和运动次数较少分别是脊髓内部和外部发生穿透性SCI小鼠的特异性表现。此外，仅在脊髓内部发生穿透性SCI的小鼠中，BMS评分与开放场地中的步行距离相关。因此，脊髓内部和外部的穿透性SCI会导致不同严重程度和进展的地面行走困难。
