Department of Regulatory Bioorganic Chemistry, SANKEN, Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan.
Comprehensive Analysis Center, SANKEN, Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan.
Chem Commun (Camb). 2024 Mar 28;60(27):3645-3648. doi: 10.1039/d4cc00544a.
The structure of the complex formed by naphthyridine carbamate dimer (NCD) binding to CGG repeat sequences in DNA, associated with fragile X syndrome, has been elucidated using N-labeled NCD and H-N HSQC. In a fully saturated state, two NCD molecules consistently bind to each CGG/CGG unit, maintaining a 1 : 2 binding stoichiometry.
使用 N 标记的 NCD 和 H-N HSQC，阐明了与脆性 X 综合征相关的 DNA 中萘啶基氨基甲酸酯二聚体（NCD）与 CGG 重复序列形成的复合物的结构。在完全饱和状态下，两个 NCD 分子始终与每个 CGG/CGG 单元结合，保持 1:2 的结合比例。
