Radiation Sciences Department, Medical Research Institute, Alexandria University, Alexandria, Egypt.
Cancer Management and Research Department, Medical Research Institute, Alexandria University, Alexandria, Egypt.
Asian Pac J Cancer Prev. 2024 Feb 1;25(2):447-452. doi: 10.31557/APJCP.2024.25.2.447.
Breast cancer (BC) is the most common form of cancer among women and the second leading cause of cancer-related death worldwide. Several malignancies can be successfully treated with radiation (RT), although radioresistance is still a major contributor to radiotherapy failure. Ionizing radiation (IR) induces pyroptosis in cancer cells. Pyroptosis is a designed method of death connected to routine immunity and directly related to the body ROS content. Objective for the study: The aim of this work was to investigate the role of serum GSDMD-CT, nucleotide-binding domain and leucine-rich-repeat-containing family pyrin 3 (NLRP3) and IL-18 as predictors of pyroptotic cell death mechanism induced by radiotherapy in breast cancer patients.
The 70 female participants in this study were divided into two groups: Group (I): 40 breast cancer patients treated with radiotherapy. Group (II): a control group of 30 healthy volunteers with similar ages and sex. Patients with breast cancer received radiation, with a dose of 44 Gray administered over the course of 16 days in five daily fractions of 2.75 Gray each. Two blood samples were taken from breast cancer patients: one before radiotherapy and the other after radiotherapy. While one blood sample was taken from healthy controls. The levels of the circulating pyroptosis biomarkers IL-18, NLRP3, and GSDMD-CT were measured using the ELISA method.
Our results showed that, there was a significant increase in serum pyroptosis markers GSDMD-CT, NLRP3 and IL-18 in BC Patients after RT when compared to before radiotherapy.
Radiotherapy induced pyroptosis in breast cancer patients as a new cell death mechanism. GSDMD-CT, NLRP3 and IL-18 are biomarkers of pyroptosis that significantly increased post irradiation highlighting enhanced ROS and pyroptosis induction.
乳腺癌（BC）是女性中最常见的癌症类型，也是全球癌症相关死亡的第二大主要原因。尽管放射抵抗仍然是放射治疗失败的主要原因，但几种恶性肿瘤可以通过放射治疗（RT）成功治疗。电离辐射（IR）会在癌细胞中诱导细胞焦亡。细胞焦亡是一种与固有免疫有关的程序性细胞死亡方式，与体内 ROS 含量直接相关。研究目的：本研究旨在探讨血清 GSDMD-CT、核苷酸结合寡聚结构域和富含亮氨酸重复序列家族 pyrin 3（NLRP3）和 IL-18 作为预测乳腺癌患者放射治疗诱导细胞焦亡机制的标志物的作用。
本研究共纳入 70 名女性参与者，分为两组：组（I）：40 名接受放射治疗的乳腺癌患者。组（II）：30 名年龄和性别相匹配的健康志愿者对照组。乳腺癌患者接受放射治疗，剂量为 44 Gray，在 16 天内每天 5 次，每次 2.75 Gray。从乳腺癌患者中采集两份血样：一份在放射治疗前，另一份在放射治疗后。从健康对照组中采集一份血样。使用 ELISA 法测量循环细胞焦亡生物标志物 IL-18、NLRP3 和 GSDMD-CT 的水平。
我们的结果表明，与放射治疗前相比，放射治疗后 BC 患者血清细胞焦亡标志物 GSDMD-CT、NLRP3 和 IL-18 显著增加。
放射治疗诱导乳腺癌患者发生细胞焦亡，这是一种新的细胞死亡机制。GSDMD-CT、NLRP3 和 IL-18 是细胞焦亡的生物标志物，放射治疗后显著增加，提示 ROS 和细胞焦亡诱导增强。
