Department of Medical Services and Techniques, Vocational School of Health Services, University of Gaziantep, Gaziantep, Turkey.
Asian Pac J Cancer Prev. 2024 Feb 1;25(2):461-464. doi: 10.31557/APJCP.2024.25.2.461.
A major part of the cytokines secreted from the immune system are interleukins (IL) and their main role is to stimulate the immune system cells. Therefore the genotypic effects of IL-6 and IL-10 on the immune system in CLL were investigated in the study.
For this purpose 100 patients diagnosed with CLL and 70 healthy individuals with no cancer history were included in the study. Polymorphisms at IL10 and IL 6 promoter regions (1082 A\G and 819 C\T) and IL6 (174 G\C) polymorphisms were analyzed with RT-PCR. Genotype and allele frequencies were directly calculated.
In 100 CLL patients, 45 wild type AA, 40 AG and 15 mutant type GG genotypes were found for the IL 10 1082 A\G region. Genotypic distribution of IL10 819 C\T region was determined as CC, BT and TT genotypes in 37, 50 and 13 patients, respectively. In IL 6 174 G\C region, GG, GC and CC genotypes were determined in 62, 30 and 8 patients, respectively. There is no statistically significant difference between the CLL patients and control groups in terms of IL10 1082 A\G, 819 C\T and IL 6 174 G/C regions (p> 0.05). As a result of the allele frequency calculation of the IL 10 1082 region, the values obtained were A (0.65), G (0.35) for the patient group and (0.61) and G (0.31) for the control group. 819 region allele frequencies were C (0.57) and T (0.33) in the patient group and C (0.48) and T (0.32) in the control group. The IL6 174 region was calculated as G (0.82), C (0.28) in the patient group and G (0.63), C (0.23) in the control group. Given the number of patients within the limits of this study, IL 10 and IL 6 genotype frequencies do not seem to be statistically related to CLL patients.
Mutant alleles of all interleukin SNPs were determined at a higher frequency in the patient group as compared to the control group. Therefore, a potential correlation between the SNPs of these interleukins and CLL can be determined in future studies with a higher number of samples.
免疫系统分泌的细胞因子中很大一部分是白细胞介素（IL），其主要作用是刺激免疫系统细胞。因此，本研究调查了白细胞介素 6（IL-6）和白细胞介素 10（IL-10）的基因型对 CLL 患者免疫系统的影响。
为此，本研究纳入了 100 名被诊断为 CLL 的患者和 70 名无癌症史的健康个体。采用 RT-PCR 分析白细胞介素 10 和白细胞介素 6 启动子区域（1082 A\G 和 819 C\T）以及白细胞介素 6（174 G\C）多态性的多态性。直接计算基因型和等位基因频率。
在 100 名 CLL 患者中，IL 10 1082 A\G 区域发现 45 个野生型 AA、40 个 AG 和 15 个突变型 GG 基因型。IL10 819 C\T 区域的基因型分布分别为 37 名患者的 CC、BT 和 TT 基因型、50 名患者的 CC、BT 和 TT 基因型和 13 名患者的 CC、BT 和 TT 基因型。在 IL 6 174 G\C 区域，GG、GC 和 CC 基因型分别确定为 62、30 和 8 名患者。在白细胞介素 10 1082 A\G、819 C\T 和白细胞介素 6 174 G/C 区域，CLL 患者与对照组之间的基因型分布无统计学差异（p>0.05）。白细胞介素 10 1082 区域的等位基因频率计算结果为患者组的 A（0.65）、G（0.35）和对照组的 A（0.61）、G（0.31）。819 区域的等位基因频率在患者组为 C（0.57）和 T（0.33），在对照组为 C（0.48）和 T（0.32）。IL6 174 区域在患者组中计算为 G（0.82）和 C（0.28），在对照组中为 G（0.63）和 C（0.23）。鉴于本研究范围内的患者数量，白细胞介素 10 和白细胞介素 6 基因型频率似乎与 CLL 患者无统计学关联。
与对照组相比，所有白细胞介素 SNP 的突变等位基因在患者组中以更高的频率存在。因此，在未来具有更高样本量的研究中，可以确定这些白细胞介素的 SNP 与 CLL 之间的潜在相关性。
