Department of Biochemistry and Biophysics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Asian Pac J Cancer Prev. 2024 Feb 1;25(2):473-483. doi: 10.31557/APJCP.2024.25.2.473.
In the majority of cancers, metastasis of tumor cells is the main cause of treatment failure. This study intended to investigate the effectiveness of basic fibroblast growth factor (bFGF) peptide designed to inhibit tumor growth in 4T1 metastatic breast cancer through the PI3K/AKT and ERK/MAPK signal transduction pathways.
The tumor was induced through 4T1 tumor graft in BALB/c mice. The designed peptide was injected intraperitoneal at three selected doses after two weeks for 14 days. The PBS and doxorubicin were used as the negative and positive control groups, respectively. Tumor size was measured and after the treatment period, the mice underwent a surgery and tumors were used for the western blot examinations.
the peptide injection was effective in reducing or inhibiting tumor growth in mice model and in vitro. The western blot analysis results showed that the p-AKT and p-ERK levels in peptide treated tumors were reduced (p<0.05).
The peptide injection was effective in mice model. Findings showed that in the two signal transduction pathways, the p-AKT and p-ERK levels were significantly different from the negative control group.
在大多数癌症中，肿瘤细胞的转移是治疗失败的主要原因。本研究旨在通过 PI3K/AKT 和 ERK/MAPK 信号转导通路，研究设计用于抑制 4T1 转移性乳腺癌肿瘤生长的碱性成纤维细胞生长因子（bFGF）肽的效果。
通过 BALB/c 小鼠的 4T1 肿瘤移植诱导肿瘤。在两周后，选择三个剂量进行腹腔注射设计的肽，持续 14 天。PBS 和阿霉素分别作为阴性和阳性对照组。测量肿瘤大小，治疗期结束后，对小鼠进行手术，使用肿瘤进行 Western blot 检查。
肽注射可有效减少或抑制小鼠模型和体外的肿瘤生长。Western blot 分析结果表明，肽处理肿瘤中的 p-AKT 和 p-ERK 水平降低（p<0.05）。
肽注射在小鼠模型中有效。研究结果表明，在两条信号转导通路中，p-AKT 和 p-ERK 水平与阴性对照组有显著差异。
