Ishimura K, Yoshinaga T, Fujita H, Sugano S, Okamoto M, Yamano T
Arch Histol Jpn. 1985 Dec;48(5):541-6. doi: 10.1679/aohc.48.541.
In order to know the mechanism of steroid hormone biosynthesis, the localization of cytochromes P-450 of cholesterol side-chain cleavage enzymes (P-450SCC) and P-450 of 11 beta-hydroxylase (P-450(11)beta) were studied in bovine adrenal glands by light as well as electron microscopic immunohistochemistry, using monoclonal antibodies. With light microscopy the cytoplasm of the glomerulosa cells was faintly immunostained, while that of the fasciculata-reticularis cells was intensely immunostained by both monoclonal antibodies for cytochrome P-450SCC and cytochrome P-450(11)beta, though the capsular connective tissue cells and the adrenal medullary cells were entirely negative for these reactions. Electron microscopic immunohistochemistry revealed that the positive reaction products for cytochromes P-450 were present on the matrix side of the inner mitochondrial membrane including the tubulovesicular cristae of the cortical cells, and especially of the fasciculata and reticularis cells. The present results indicate that both cholesterol side-chain cleaving enzymes and 11 beta-hydroxylase are present in the inner mitochondrial membrane of bovine adrenal cortical cells.
为了解甾体激素生物合成的机制，利用单克隆抗体，通过光镜及电镜免疫组织化学方法，对牛肾上腺中胆固醇侧链裂解酶细胞色素P - 450（P - 450SCC）和11β - 羟化酶细胞色素P - 450（P - 450(11)β）进行定位研究。光镜下，球状带细胞的胞质呈弱阳性免疫染色，而束状带 - 网状带细胞的胞质被细胞色素P - 450SCC和细胞色素P - 450(11)β的单克隆抗体强烈免疫染色，尽管被膜结缔组织细胞和肾上腺髓质细胞对这些反应完全呈阴性。电镜免疫组织化学显示，细胞色素P - 450的阳性反应产物存在于皮质细胞，尤其是束状带和网状带细胞的线粒体内膜基质侧，包括管状泡状嵴。目前的结果表明，胆固醇侧链裂解酶和11β - 羟化酶都存在于牛肾上腺皮质细胞的线粒体内膜。
