Sasano H, Okamoto M, Sasano N
Department of Pathology, Tohoku University School of Medicine, Sendai, Japan.
Virchows Arch A Pathol Anat Histopathol. 1988;413(4):313-8. doi: 10.1007/BF00783023.
Cytochrome P-450 specific for steroid 11 beta-hydroxylation (P-45011 beta) was immunohistochemically demonstrated in the adrenal glands of human, pig and bovine and of mineralo- and glucocorticoid excess using a specific monoclonal antibody against P-450 11 beta of bovine adrenocortical mitochondria. P-450 11 beta was present in all three cortical zones of the histologically normal adrenal glands of bovine, pig and human, particularly in the zona fasciculata (ZF) and reticularis (ZR). The P-450 11 beta immunoreactivity was intensive in cortical micronodules and inner ZF and ZR in Cushing's disease, and relatively intensive in the zona glomerulosa (ZG) and outer ZF in idiopathic hyperaldosteronism (IHA), corresponding to the sites of active steroidogenesis. In adenomas with Cushing's syndrome and primary aldosteronism, compact cells were generally stained well. In the adrenal glands attached to the adenomas, immunoreactivity was observed only focally in ZG cells but not in ZF and ZR cells.
利用针对牛肾上腺皮质线粒体P-450 11β的特异性单克隆抗体，通过免疫组织化学方法在人、猪和牛的肾上腺以及盐皮质激素和糖皮质激素过多的肾上腺中证实了对类固醇11β-羟化具有特异性的细胞色素P-450（P-45011β）。P-450 11β存在于牛、猪和人的组织学正常肾上腺的所有三个皮质带中，尤其在束状带（ZF）和网状带（ZR）中。在库欣病中，P-450 11β免疫反应性在皮质微结节以及ZF和ZR内层中较强，而在特发性醛固酮增多症（IHA）中，在球状带（ZG）和ZF外层中相对较强，这与活跃的类固醇生成部位相对应。在患有库欣综合征和原发性醛固酮增多症的腺瘤中，致密细胞通常染色良好。在与腺瘤相连的肾上腺中，仅在ZG细胞中局部观察到免疫反应性，而在ZF和ZR细胞中未观察到。
