白藜芦醇在人真皮乳头细胞中显示出对 PM2.5 诱导的细胞衰老的实验细胞模型的有力衰老逆转作用。
Resveratrol Shows Potent Senescence Reversal in Experimental Cellular Models of Particular Matter 2.5-induced Cellular Senescence in Human Dermal Papilla Cells.
机构信息
Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand.
Center of Excellence in Cancer Cell and Molecular Biology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand.
出版信息
In Vivo. 2024 Mar-Apr;38(2):665-673. doi: 10.21873/invivo.13487.
BACKGROUND/AIM: Particular matter 2.5 (PM2.5) pollution is associated with senescence induction. Since the impact of PM2.5 on stem cell senescence and potential compounds capable of reversing this process are largely unknown, this study aimed to examine the senescence effects of PM2.5 on dermal papilla (DP) stem cells. Additionally, we explored the reversal of these effects using natural product-derived substances, such as resveratrol (Res) or Emblica fruits, soybean, and Thunbergia Laurifolia (EST) extract.
MATERIALS AND METHODS
Cell senescence was determined using the β-Galactosidase (SA-β-gal) assay. The senescence-associated secretory phenotype (SASP) was detected using real-time RT-PCR. For senescence markers, the mRNA and protein levels of p21 and p16 were measured using real-time RT-PCR and immunofluorescence analysis.
RESULTS
Subtoxic concentration of PM2.5 (50 μg/ml) induced senescence in DP cells. Resveratrol (50, 100 μM) and plant extracts (400, 800 μg/ml) reversed PM2.5-induced cell senescence. Treatment with Res or EST significantly decreased SA-β-gal staining in PM2.5-treated cells. Furthermore, Res and EST decreased the mRNA levels of SASP, including IL1α, IL7, IL8, and CXCL1. DP cells exposed to PM2.5 exhibited an increase in p21 and p16 mRNA and protein levels, which could be reversed by the addition of Res or EST. Res and EST could reduce p21 and p16 in senescent cells approximately 3- and 2-fold, respectively, compared to untreated senescent cells.
CONCLUSION
PM2.5 induced senescence in human DP stem cells. Res and EST extract potentially reverse the senescence phenotypes of such cells.
背景/目的：细颗粒物 2.5（PM2.5）污染与衰老诱导有关。由于 PM2.5 对干细胞衰老的影响以及潜在的能够逆转这一过程的化合物在很大程度上尚不清楚，因此本研究旨在检查 PM2.5 对真皮乳头（DP）干细胞衰老的影响。此外，我们还探索了使用天然产物衍生的物质（如白藜芦醇（Res）或余甘子、大豆和 Thunbergia Laurifolia（EST）提取物）逆转这些效应。
材料和方法
通过β-半乳糖苷酶（SA-β-gal）测定法确定细胞衰老。通过实时 RT-PCR 检测衰老相关分泌表型（SASP）。对于衰老标志物，使用实时 RT-PCR 和免疫荧光分析测量 p21 和 p16 的 mRNA 和蛋白水平。
结果
亚毒性浓度的 PM2.5（50μg/ml）诱导 DP 细胞衰老。白藜芦醇（50、100μM）和植物提取物（400、800μg/ml）逆转了 PM2.5 诱导的细胞衰老。Res 或 EST 处理显著降低了 PM2.5 处理细胞中的 SA-β-gal 染色。此外，Res 和 EST 降低了 SASP 的 mRNA 水平，包括 IL1α、IL7、IL8 和 CXCL1。暴露于 PM2.5 的 DP 细胞中 p21 和 p16 的 mRNA 和蛋白水平增加，而 Res 或 EST 的添加可逆转这一现象。与未处理的衰老细胞相比，Res 和 EST 可使衰老细胞中的 p21 和 p16 分别减少约 3 倍和 2 倍。
结论
PM2.5 诱导人 DP 干细胞衰老。Res 和 EST 提取物可能逆转这些细胞的衰老表型。
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