Epigallocatechin Gallate Effectively Affects Senescence and Anti-SASP via in 3T3-L1 Preadipocytes in Comparison with Other Bioactive Substances.

AIM

We investigated different bioactive compounds including epigallocatechin gallate (EGCG), anthocyanidin, resveratrol, phloretin, spermidine, butyrate, and -hydroxybutyrate with regard to their effect on via and modulation of the proinflammatory senescence-associated secretory phenotype (SASP) in senescence induced 3T3-L1 preadipocytes.

METHODS

For induction of senescence, 3T3-L1 preadipocytes were incubated with bromodeoxyuridine (BrdU) for 8 days. Cell cycle inhibition was observed, and -galactosidase activity was measured. After BrdU treatment, cells were treated with different bioactive compounds in various concentrations for 96 h. ELISA was used for determining proinflammatory cytokine IL6 in SASP cells.

RESULTS

increased significantly after BrdU incubation compared to untreated control ( < 0.01). All secondary plant ingredients used for treatment, but not anthocyanidin 50 M, decrease expression ( < 0.05), whereas most endogenous substances did not attenuate . IL6 secretion positively correlated with ( < 0.01), whereas EGCG could diminish both, IL6 and with the strongest effect ( < 0.01). Although positively correlated with activation ( < 0.05), only resveratrol ( < 0.01) and anthocyanidin ( < 0.05) could activate significantly. Solely anthocyanidin 50 M ( < 0.05) and 100 M ( < 0.01) and EGCG 50 M ( < 0.01) could increase expression. Activation of with EGCG correlated with lowered IL6 secretion significantly ( < 0.05) but not with anthocyanidin.

CONCLUSION

Accumulation of senescent cells in adipose tissue plays an important role in obesity and age-related diseases. , located in the mitochondria, can regulate ROS via different pathways. Thus, targeting activating compounds such as EGCG may delay senescence of cells and senescence induced inflammatory processes.