使用8微秒激光脉冲在离体猪眼中进行实时光学相干断层扫描（OCT）反馈控制的视网膜色素上皮（RPE）光破坏。
Real-time OCT feedback-controlled RPE photodisruption in ex vivo porcine eyes using 8 microsecond laser pulses.
作者信息
Burri Christian, Salzmann Simon, Wandel Jasmin, Hoffmann Leonie, Považay Boris, Meier Christoph, Frenz Martin
机构信息
optoLab, Institute for Human Centered Engineering, Bern University of Applied Sciences, Biel, Switzerland.
Biomedical Photonics Group, Institute of Applied Physics, University of Bern, Bern, Switzerland.
出版信息
Biomed Opt Express. 2023 Nov 20;14(12):6328-6349. doi: 10.1364/BOE.503941. eCollection 2023 Dec 1.
Selective retinal pigment epithelium (RPE) photodisruption requires reliable real-time feedback dosimetry (RFD) to prevent unwanted overexposure. In this study, optical coherence tomography (OCT) based RFD was investigated in ex vivo porcine eyes exposed to laser pulses of 8 µs duration (wavelength: 532 nm, exposure area: 90 × 90 µm, radiant exposure: 247 to 1975 mJ/µm). For RFD, fringe washouts in time-resolved OCT M-scans (central wavelength: 870 nm, scan rate: 85 kHz) were compared to an RPE cell viability assay. Statistical analysis revealed a moderate correlation between RPE lesion size and applied treatment energy, suggesting RFD adaptation to inter- and intraindividual RPE pigmentation and ocular transmission.
选择性视网膜色素上皮（RPE）光破坏需要可靠的实时反馈剂量测定法（RFD）来防止不必要的过度曝光。在本研究中，对暴露于持续时间为8微秒的激光脉冲（波长：532 nm，暴露面积：90×90 μm，辐射曝光：247至1975 mJ/μm）的离体猪眼进行了基于光学相干断层扫描（OCT）的RFD研究。对于RFD，将时间分辨OCT M扫描（中心波长：870 nm，扫描速率：85 kHz）中的条纹消失与RPE细胞活力测定进行比较。统计分析显示RPE病变大小与应用的治疗能量之间存在中度相关性，表明RFD可适应个体间和个体内的RPE色素沉着及眼内透射。
Zotero 插件