• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一种用于评估牛至纯度的数字PCR方法。

A digital PCR approach to assess the purity of oregano.

作者信息

Cottenet Geoffrey, Blancpain Carine, Holzwarth James

机构信息

Institute of Food Safety & Analytical Sciences, Nestlé Research, Vers-chez-les-Blanc, Lausanne, Switzerland.

出版信息

Heliyon. 2024 Feb 11;10(4):e25985. doi: 10.1016/j.heliyon.2024.e25985. eCollection 2024 Feb 29.

DOI:10.1016/j.heliyon.2024.e25985
PMID:38420487
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10900423/
Abstract

Herbs and spices are food categories known to be at high risk of adulteration. Presence of undeclared foreign plant species has often been reported in oregano and may have a direct impact on its organoleptic quality and potentially the safety of this aromatic herb. A droplet digital PCR approach was developed to assess the purity of oregano by quantifying the DNA copies of oregano versus the total plant DNA copies. Nuclear single-copy genes were selected by targeting the gene from oregano and the plant gene. The reactions were specific to the Origanum genus and plant materials respectively, whereas trueness and precision data confirmed the reliability of the method to quantify oregano. The applicability of the method was further verified on proficiency test samples before being applied on commercial oregano samples.

摘要

草药和香料是已知极易掺假的食品类别。牛至中经常被报道含有未申报的外来植物物种，这可能会直接影响其感官品质，并可能影响这种香草的安全性。开发了一种液滴数字PCR方法，通过量化牛至的DNA拷贝数与植物总DNA拷贝数来评估牛至的纯度。通过靶向牛至的基因和植物基因来选择核单拷贝基因。这些反应分别对牛至属和植物材料具有特异性，而准确性和精密度数据证实了该方法量化牛至的可靠性。在将该方法应用于商业牛至样品之前，先在能力验证样品上进一步验证了该方法的适用性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5d5/10900423/500b1d901ff8/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5d5/10900423/81ff305c1b4d/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5d5/10900423/bf864ff6a132/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5d5/10900423/500b1d901ff8/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5d5/10900423/81ff305c1b4d/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5d5/10900423/bf864ff6a132/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5d5/10900423/500b1d901ff8/gr3.jpg

相似文献

1
A digital PCR approach to assess the purity of oregano.一种用于评估牛至纯度的数字PCR方法。
Heliyon. 2024 Feb 11;10(4):e25985. doi: 10.1016/j.heliyon.2024.e25985. eCollection 2024 Feb 29.
2
Detection and Quantification of Botanical Impurities in Commercial Oregano () Using Metabarcoding and Digital PCR.利用元条形码技术和数字PCR检测及定量商品牛至中的植物性杂质()
Foods. 2023 Aug 9;12(16):2998. doi: 10.3390/foods12162998.
3
A comprehensive strategy to detect the fraudulent adulteration of herbs: The oregano approach.一种检测草药掺假欺诈的综合策略：牛至方法。
Food Chem. 2016 Nov 1;210:551-7. doi: 10.1016/j.foodchem.2016.05.004. Epub 2016 May 3.
4
Critical evaluation of ambient mass spectrometry coupled with chemometrics for the early detection of adulteration scenarios in Origanum vulgare L.结合化学计量学的环境质谱法用于牛至掺假情况早期检测的批判性评估
Talanta. 2021 May 15;227:122116. doi: 10.1016/j.talanta.2021.122116. Epub 2021 Feb 8.
5
A DNA Metabarcoding Workflow to Identify Species in Spices and Herbs.一种用于鉴定香料和草药中物种的DNA代谢条形码工作流程。
J AOAC Int. 2022 Dec 22;106(1):65-72. doi: 10.1093/jaoacint/qsac099.
6
The origin of in-vitro estrogen-like activity in oregano herb extracts.牛至草提取物中具有体外雌激素样活性物质的起源。
Toxicol In Vitro. 2019 Apr;56:101-109. doi: 10.1016/j.tiv.2019.01.005. Epub 2019 Jan 11.
7
Creation of New Oregano Genotypes with Different Terpene Chemotypes via Inter- and Intraspecific Hybridization.通过种间和种内杂交创建具有不同萜烯化学型的新型牛至基因型。
Int J Mol Sci. 2023 Apr 15;24(8):7320. doi: 10.3390/ijms24087320.
8
Plasticizers and BPA in spices and aromatic herbs of Mediterranean areas.地中海地区香料和芳香草药中的增塑剂和双酚 A。
Nat Prod Res. 2020 Jan;34(1):87-92. doi: 10.1080/14786419.2019.1591403. Epub 2019 Mar 24.
9
A comprehensive study on the phenolic profile of widely used culinary herbs and spices: rosemary, thyme, oregano, cinnamon, cumin and bay.广泛使用的烹饪香草和香料的酚类成分的综合研究：迷迭香、百里香、牛至、肉桂、孜然和月桂。
Food Chem. 2014 Jul 1;154:299-307. doi: 10.1016/j.foodchem.2013.12.106. Epub 2014 Jan 8.
10
Influence of oregano essential oil on traditional Argentinean cheese elaboration: Effect on lactic starter cultures.牛至精油对阿根廷传统奶酪制作的影响：对乳酸发酵剂的作用。
Rev Argent Microbiol. 2016 Jul-Sep;48(3):229-235. doi: 10.1016/j.ram.2016.04.006. Epub 2016 Aug 28.

本文引用的文献

1
Monoterpene synthases of three closely related sage species (Salvia officinalis, S. fruticosa and S. pomifera, Lamiaceae).三种近缘鼠尾草属植物（药用鼠尾草、灌木鼠尾草和石榴鼠尾草，唇形科）的单萜合酶
Plant Physiol Biochem. 2023 Mar;196:318-327. doi: 10.1016/j.plaphy.2023.01.034. Epub 2023 Jan 19.
2
A DNA Metabarcoding Workflow to Identify Species in Spices and Herbs.一种用于鉴定香料和草药中物种的DNA代谢条形码工作流程。
J AOAC Int. 2022 Dec 22;106(1):65-72. doi: 10.1093/jaoacint/qsac099.
3
Food Tracking Perspective: DNA Metabarcoding to Identify Plant Composition in Complex and Processed Food Products.
食品追踪视角：DNA 代谢组学鉴定复杂加工食品中的植物成分。
Genes (Basel). 2019 Mar 25;10(3):248. doi: 10.3390/genes10030248.
4
Performance assessment of digital PCR for the quantification of GM-maize and GM-soya events.数字 PCR 定量检测 GM 玉米和 GM 大豆事件的性能评估。
Anal Bioanal Chem. 2019 Apr;411(11):2461-2469. doi: 10.1007/s00216-019-01692-7. Epub 2019 Feb 27.
5
Overview of Analytical Tools for the Identification of Adulterants in Commonly Traded Herbs and Spices.常见贸易草药和香料中掺假物鉴定分析工具概述
J AOAC Int. 2019 Mar 1;102(2):376-385. doi: 10.5740/jaoacint.18-0389. Epub 2019 Jan 15.
6
Study on Commercial Spice and Herb Products Using Next-Generation Sequencing (NGS).利用新一代测序技术（NGS）对商业香料和草药产品的研究。
J AOAC Int. 2019 Mar 1;102(2):369-375. doi: 10.5740/jaoacint.18-0407. Epub 2019 Jan 4.
7
Critical assessment of digital PCR for the detection and quantification of genetically modified organisms.对数字 PCR 检测和定量遗传修饰生物体的批判性评估。
Anal Bioanal Chem. 2018 Jul;410(17):4039-4050. doi: 10.1007/s00216-018-1010-1. Epub 2018 Mar 24.
8
A comprehensive strategy to detect the fraudulent adulteration of herbs: The oregano approach.一种检测草药掺假欺诈的综合策略：牛至方法。
Food Chem. 2016 Nov 1;210:551-7. doi: 10.1016/j.foodchem.2016.05.004. Epub 2016 May 3.
9
Measuring Digital PCR Quality: Performance Parameters and Their Optimization.测量数字PCR质量：性能参数及其优化
PLoS One. 2016 May 5;11(5):e0153317. doi: 10.1371/journal.pone.0153317. eCollection 2016.
10
Pitfalls of DNA Quantification Using DNA-Binding Fluorescent Dyes and Suggested Solutions.使用DNA结合荧光染料进行DNA定量的陷阱及建议解决方案
PLoS One. 2016 Mar 3;11(3):e0150528. doi: 10.1371/journal.pone.0150528. eCollection 2016.