School of Materials and Chemistry, University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai, China; Key Laboratory of Inorganic Coating Materials CAS, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, China.
Key Laboratory of Inorganic Coating Materials CAS, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, China; Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, China.
Colloids Surf B Biointerfaces. 2024 Apr;236:113825. doi: 10.1016/j.colsurfb.2024.113825. Epub 2024 Feb 25.
Dynamical control of macrophage polarization from M1 (pro-inflammatory) to M2 (anti-inflammatory) at implant surfaces is essential for balancing innate immunity and tissue repair. In this aspect, the design of orthopedic implant that can response to inflammation microenvironment with transformation in surface properties has shown promising in timely driving M1-to-M2 macrophage transition. Considering excessive reactive oxygen species (ROS) contribute to macrophage M1 polarization and progression of inflammation, in this study, ferrocene modified polydopamine (PDA-Fc) films were deposited on plasma sprayed Ti coatings to endow the implants with ROS-responsive and -scavenging abilities. Plasma sprayed Ti (PST) coating and PDA modified PST coating (PST/PDA) served as control. The presence of PDA endowed PST/PDA and PST/PDA-Fc with free-radical scavenging abilities. Moreover, PST/PDA-Fc showed adaptive wettability as evidenced by increased hydrophilicity under HO treatment. With respect to PST/PDA, PST/PDA-Fc exerted greater effects on inducing lipopolysaccharides-induced M1 macrophages to adopt M2-type macrophage phenotype, characterized by higher percentage of CD206-positive cells, increased cell elongation rate and higher expression level of anti-inflammatory cytokine arginase type 1. The results obtained in our study may provide a prospective approach for manipulating an appropriate immune response at implant surfaces.
在植入物表面将巨噬细胞从 M1（促炎）极化到 M2（抗炎）的动力学控制对于平衡先天免疫和组织修复至关重要。在这方面，设计能够响应炎症微环境并在表面性质上发生转变的骨科植入物已显示出在及时驱动 M1 向 M2 巨噬细胞转化方面的巨大潜力。考虑到过量的活性氧（ROS）有助于巨噬细胞 M1 极化和炎症的进展，在本研究中，将二茂铁修饰的聚多巴胺（PDA-Fc）薄膜沉积在等离子喷涂 Ti 涂层上，使植入物具有 ROS 响应和清除能力。等离子喷涂 Ti（PST）涂层和 PDA 修饰的 PST 涂层（PST/PDA）作为对照。PDA 的存在赋予了 PST/PDA 和 PST/PDA-Fc 自由基清除能力。此外，PST/PDA-Fc 表现出适应性润湿性，HO 处理下亲水性增加。与 PST/PDA 相比，PST/PDA-Fc 对诱导脂多糖诱导的 M1 巨噬细胞向 M2 型巨噬细胞表型的诱导作用更大，表现为 CD206 阳性细胞的比例更高、细胞伸长率更高和抗炎细胞因子精氨酸酶 1 的表达水平更高。我们的研究结果可能为在植入物表面操纵适当的免疫反应提供一种有前景的方法。
