Karthika Palanisamy, Shanmuganathan Saravanakumar, Subramanian Viswanathan, Delerue-Matos Cristina
Department of Industrial Chemistry, Alagappa University, Karaikudi, 630 003, Tamil Nadu, India.
Department of Industrial Chemistry, Alagappa University, Karaikudi, 630 003, Tamil Nadu, India.
Talanta. 2024 May 15;272:125823. doi: 10.1016/j.talanta.2024.125823. Epub 2024 Feb 22.
A novel electrochemical sensor was developed for the detection of salivary cortisol levels. The sensor employs a combination of a molecularly imprinted polymer (MIP) and gold nanoparticles (AuNPs) that are electrodeposited onto a screen-printed electrode (SPE). The study utilised density functional theory and molecular docking techniques to determine the geometry of molecular orbitals, electrostatic potential energies, and binding energy of cortisol and the polymers. The thin film of cortisol-imprinted polymer on the SPE was created by electro-polymerizing pyrrole and thiophene-3-carboxylic acid on the electrode surface along with cortisol as the template molecule. The MIP film was characterised using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and electrochemical techniques. The sensor exhibited a linear response in the concentration range of 0.05 nmol L to 2.5 μmol L, with a limit of detection of 0.01 nmol L, as determined by differential pulse voltammetry. This method offers a simple yet efficient and sensitive approach to detecting cortisol levels in human saliva samples.
开发了一种用于检测唾液皮质醇水平的新型电化学传感器。该传感器采用分子印迹聚合物（MIP）和金纳米颗粒（AuNP）的组合，它们被电沉积到丝网印刷电极（SPE）上。该研究利用密度泛函理论和分子对接技术来确定皮质醇与聚合物的分子轨道几何形状、静电势能和结合能。通过在电极表面将吡咯和噻吩 - 3 - 羧酸与皮质醇作为模板分子进行电聚合，在SPE上制备了皮质醇印迹聚合物薄膜。使用扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）和电化学技术对MIP薄膜进行了表征。通过差分脉冲伏安法测定，该传感器在0.05 nmol/L至2.5 μmol/L的浓度范围内呈现线性响应，检测限为0.01 nmol/L。该方法为检测人类唾液样本中的皮质醇水平提供了一种简单、高效且灵敏的方法。
