Turk Fatih, Yildirim-Tirgil Nimet
Metallurgical & Materials Engineering Department, Faculty of Engineering & Natural Sciences, Ankara Yıldırım Beyazıt University, Ankara, 06010, Turkey.
Biomedical Engineering Department, Faculty of Engineering and Natural Sciences, Ankara Yildirim Beyazit University, Ankara, 06010, Turkey.
Bioanalysis. 2024 Mar;16(6):331-345. doi: 10.4155/bio-2023-0203. Epub 2024 Mar 1.
Investigating molecularly imprinted polymers (MIPs) in electrochemical biosensors for thrombin detection, an essential protein biomarker. Comparing different monomers to showcase distinct sensitivity, specificity and stability advantages. Dopamine, thionine and ethanolamine serve as monomers for MIP synthesis. Electrochemical methods and atomic force microscopy characterize sensor surfaces. Performance is evaluated, emphasizing monomer-specific electrochemical responses. Monomer-specific electrochemical responses highlight dopamine's superior signal change and stability over 30 days. Notably, a low 5 pg/ml limit of detection, a broad linear range (5-200 pg/ml) and enhanced selectivity against interferents are observed. Dopamine-based MIPs show promise for high-performance electrochemical thrombin biosensors, suggesting significant applications in clinical diagnostics.
研究用于凝血酶检测的电化学生物传感器中的分子印迹聚合物（MIP），凝血酶是一种重要的蛋白质生物标志物。比较不同单体以展示独特的灵敏度、特异性和稳定性优势。多巴胺、硫堇和乙醇胺用作MIP合成的单体。采用电化学方法和原子力显微镜对传感器表面进行表征。评估性能，强调单体特异性的电化学响应。单体特异性的电化学响应突出了多巴胺在30天内具有卓越的信号变化和稳定性。值得注意的是，检测限低至5 pg/ml，线性范围宽（5 - 200 pg/ml），并且对干扰物具有增强的选择性。基于多巴胺的MIP在高性能电化学凝血酶生物传感器方面显示出前景，表明在临床诊断中具有重要应用。
