Department of Pharmacy, JiangXi University of Chinese Medicine, Nanchang, JiangXi 330004, China.
School of Materials Science and Engineering, Kunming University of Science and Technology, Kunming, YunNan 650031, China.
Analyst. 2024 Mar 25;149(7):2114-2121. doi: 10.1039/d3an02237d.
In this study, we innovatively synthesized bipyridine ruthenium cluster nanosheets (RuMOFNCs), a novel metal-organic framework material that exhibits both fluorescence and electrochemiluminescence. Gold nanoparticles (AuNPs) were anchored onto RuMOFNCs bipyridine chelation, enhancing optical signals and creating sites for attaching biologically functional probes. We employed tetraferrocene-modified DNA probes, linked gold-sulfur (Au-S) bonds, to construct a dual-mode fluorescence-electrochemiluminescence biosensor. This sensor, exploiting exonuclease III (Exo III)-mediated cyclic amplification, inhibits the electron transfer from RuMOFNC to tetraferrocene, resulting in amplified fluorescence and electrochemiluminescence signals. The sensor demonstrates exceptional sensitivity for detecting the BRAF gene, with fluorescence and electrochemiluminescence detection limits of 10.3 aM (range: 0.1 fM to 1 nM) and 3.1 aM (range: 1 aM to 10 pM), respectively. These capabilities are attributed to RuMOFNCs' luminescence properties, tetraferrocene's quenching effect, and the specificity of base pairing. This study's findings hold substantial promise for biomedical research and clinical diagnostics, particularly in precision medicine and early disease detection.
在本研究中，我们创新性地合成了联吡啶钌簇纳米片（RuMOFNCs），这是一种新颖的金属有机框架材料，具有荧光和电致化学发光两种性质。金纳米粒子（AuNPs）通过联吡啶螯合作用锚定在 RuMOFNCs 上，增强了光学信号，并为附着生物功能探针创造了位点。我们采用四茂铁修饰的 DNA 探针，通过金-硫（Au-S）键连接，构建了一种双模荧光-电化学发光生物传感器。该传感器利用外切酶 III（Exo III）介导的循环扩增，抑制了 RuMOFNC 向四茂铁的电子转移，从而产生了放大的荧光和电化学发光信号。该传感器对 BRAF 基因的检测具有出色的灵敏度，荧光和电化学发光检测限分别为 10.3 aM（范围：0.1 fM 至 1 nM）和 3.1 aM（范围：1 aM 至 10 pM）。这些性能归因于 RuMOFNCs 的发光性质、四茂铁的猝灭效应以及碱基配对的特异性。本研究的结果为生物医学研究和临床诊断提供了很大的应用前景，特别是在精准医学和早期疾病检测方面。
