Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology (IHBT), Palampur, 176061, HP, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India.
Appl Microbiol Biotechnol. 2024 Mar 1;108(1):247. doi: 10.1007/s00253-024-13076-8.
Host and tissue-specificity of endophytes are important attributes that limit the endophyte application on multiple crops. Therefore, understanding the endophytic composition of the targeted crop is essential, especially for the dioecious plants where the male and female plants are different. Here, efforts were made to understand the endophytic bacterial composition of the dioecious Siraitia grosvenorii plant using 16S rRNA amplicon sequencing. The present study revealed the association of distinct endophytic bacterial communities with different parts of male and female plants. Roots of male and female plants had a higher bacterial diversity than other parts of plants, and the roots of male plants had more bacterial diversity than the roots of female plants. Endophytes belonging to the phylum Proteobacteria were abundant in all parts of male and female plants except male stems and fruit pulp, where the Firmicutes were most abundant. Class Gammaproteobacteria predominated in both male and female plants, with the genus Acinetobacter as the most dominant and part of the core microbiome of the plant (present in all parts of both, male and female plants). The presence of distinct taxa specific to male and female plants was also identified. Macrococcus, Facklamia, and Propionibacterium were the distinct genera found only in fruit pulp, the edible part of S. grosvenorii. Predictive functional analysis revealed the abundance of enzymes of secondary metabolite (especially mogroside) biosynthesis in the associated endophytic community with predominance in roots. The present study revealed bacterial endophytic communities of male and female S. grosvenorii plants that can be further explored for monk fruit cultivation, mogroside production, and early-stage identification of male and female plants. KEY POINTS: • Male and female Siraitia grosvenorii plants had distinct endophytic communities • The diversity of endophytic communities was specific to different parts of plants • S. grosvenorii-associated endophytes may be valuable for mogroside biosynthesis and monk fruit cultivation.
雌雄异株植物的宿主和组织特异性是限制其在多种作物中应用的重要属性。因此，了解目标作物的内生菌组成是必要的，特别是对于雌雄异株植物，其雄株和雌株是不同的。在这里，我们努力使用 16S rRNA 扩增子测序来了解雌雄异株罗汉果植物的内生细菌组成。本研究揭示了不同部分的雌雄异株植物与不同的内生细菌群落相关联。雌雄株植物的根比其他部分具有更高的细菌多样性，而雄株的根比雌株的根具有更多的细菌多样性。除了雄株茎和果实果肉外，所有雌雄株植物的内共生菌都属于变形菌门，而厚壁菌门是最丰富的。在雌雄株植物中，γ变形菌纲都占优势，其中不动杆菌属是最主要的，也是植物核心微生物组的一部分（存在于雌雄株植物的所有部分）。还鉴定了雌雄株植物特有的独特分类群。在罗汉果的可食用部分（果肉）中，仅发现了 macrosoccus、facklamia 和丙酸杆菌等独特属。预测功能分析显示，与内生群落相关的次级代谢产物（特别是罗汉果苷）生物合成酶的丰度较高，而内生群落主要存在于根部。本研究揭示了雌雄罗汉果植物的内生细菌群落，这些群落可进一步用于罗汉果的栽培、罗汉果苷的生产以及雌雄株植物的早期鉴定。关键点： • 雌雄异株罗汉果植物具有不同的内生菌群 • 内生菌群的多样性与植物的不同部位有关 • 与罗汉果相关的内生菌可能对罗汉果苷生物合成和罗汉果栽培有价值。
