Neurobiology Group, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.
Methods Mol Biol. 2024;2761:231-243. doi: 10.1007/978-1-0716-3662-6_16.
Microglia are scavengers of the brain environment that clear dead cells, debris, and microbes. In Alzheimer's disease, microglia get activated to phagocytose damaged neurons, extracellular Amyoid-β, and Tau deposits. Several Tau internalization mechanisms of microglia have been studied which include phagocytosis, pinocytosis, and receptor-mediated endocytosis. In this chapter, we have visualized microglial phagocytic structures that are actin-rich cup-like extensions, which surrounds extracellular Tau species by wide-field fluorescence and confocal microscopy. We have shown the association of filamentous actin in Tau phagocytosis along the assembly of LC-3 molecules to phagosomes. The 3-dimensional, orthogonal and gallery wise representation of these phagocytic structures provides an overview of the phagocytic mechanism of extracellular Tau by microglia.
小胶质细胞是大脑环境中的清道夫，可清除死亡细胞、碎片和微生物。在阿尔茨海默病中，小胶质细胞被激活以吞噬受损的神经元、细胞外淀粉样β和 Tau 沉积物。已经研究了小胶质细胞摄取 Tau 的几种内在机制，包括吞噬作用、胞饮作用和受体介导的内吞作用。在本章中，我们通过宽场荧光和共聚焦显微镜观察到了小胶质细胞吞噬性结构，这些结构是富含肌动蛋白的杯状延伸物，可通过宽场荧光和共聚焦显微镜围绕细胞外 Tau 物种形成。我们已经显示了在 LC-3 分子组装到吞噬体的过程中，丝状肌动蛋白在 Tau 吞噬作用中的关联。这些吞噬结构的三维、正交和画廊式表示提供了小胶质细胞吞噬细胞外 Tau 的机制概述。
