Moradabad Educational Trust Group of Institutions Faculty of Pharmacy, Moradabad, Uttar Pradesh, India.
ISF College of Pharmacy, Moga, Punjab, India.
Methods Mol Biol. 2024;2761:499-510. doi: 10.1007/978-1-0716-3662-6_34.
Trichloroethylene, a chlorinated solvent widely used as a degreasing agent, is a common environmental contaminant. Emerging evidence suggests that chronic exposure to trichloroethylene (TCE) contributes to the development of Parkinson's disease (PD). TCE induced LRRK2 kinase activity in the rat brain and produced a significant dopaminergic lesion in the nigrostriatal tract with elevated oxidative stress. Here we have utilized TCE-induced PD model for the assessment of test drug. Oral gavage administration of TCE at a dose of 1000 mg/kg/day for 6 weeks was utilized to induced PD. Muscle grip strength was estimated by rotarod and grid performance test. Motor activity by actophotometer and locomotor stability were assessed by forelimb locomotor scale (FLS) and forelimb step alternation test (FSAT). However, the postural stability was assessed by postural stability test (PST). Biochemical estimation consists of determination of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), GSH level (reduced glutathione), and nitrite concentration.
三氯乙烯，一种广泛用作脱脂剂的氯化溶剂，是一种常见的环境污染物。新出现的证据表明，慢性接触三氯乙烯（TCE）会导致帕金森病（PD）的发展。TCE 在大鼠大脑中诱导 LRRK2 激酶活性，并在黑质纹状体束中产生显著的多巴胺能损伤，同时伴有氧化应激升高。在这里，我们利用 TCE 诱导的 PD 模型来评估测试药物。通过灌胃给予 TCE 剂量为 1000mg/kg/天，持续 6 周，以诱导 PD。通过转棒和网格性能测试来评估肌肉握力。通过活动计和前肢运动量表（FLS）和前肢交替踏步测试（FSAT）来评估运动活动和运动稳定性。然而，通过姿势稳定性测试（PST）来评估姿势稳定性。生化评估包括测定丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、GSH 水平（还原型谷胱甘肽）和亚硝酸盐浓度。
