超氧化物歧化酶活性测定：条件的微小变化带来的一些重大影响。
Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions.
作者信息
Beyer W F, Fridovich I
出版信息
Anal Biochem. 1987 Mar;161(2):559-66. doi: 10.1016/0003-2697(87)90489-1.
PMID:3034103
Abstract
Most assays for superoxide dismutase depend upon competition between the enzyme and some indicating scavenger for O-2. We have investigated the effects of experimental variables on assays based upon the use of either ferricytochrome c or nitro blue tetrazolium. Our results should help investigators to avoid the numerous potential pitfalls which necessarily surround these assay methods.
摘要
大多数超氧化物歧化酶检测方法依赖于该酶与某种O₂指示性清除剂之间的竞争。我们研究了实验变量对基于使用铁氰化细胞色素c或硝基蓝四唑的检测方法的影响。我们的结果应有助于研究人员避免这些检测方法必然存在的众多潜在陷阱。
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