Laboratory for Stem Cell & Restorative Neurology, Department of Biotechnology, Era's Lucknow Medical College and Hospital, Era University, Lucknow, India.
Department of Stem Cell Biology and Regenerative Medicine, Era's Lucknow Medical College Hospital, Era University, Lucknow, India.
Methods Mol Biol. 2024;2761:559-567. doi: 10.1007/978-1-0716-3662-6_37.
Spinal cord injury (SCI) is a devastating clinical condition that affects millions of people worldwide. SCI primarily affects males in younger age groups. It is characterized by a complex of neurological dysfunctions that can lead to permanent disability. We describe an adapted technique for SCI, i.e., a contusion model of SCI, in this chapter. This model is widely used to study the pathology of SCI and test potential therapies. The experimental contusion is performed by using a compression device, which allows the creation of a reproducible injury animal model through the definition of specific injury parameters. A detailed methodology has been developed and described here that utilizes a stereotactic frame and impactor to produce reproducible injuries.
脊髓损伤（SCI）是一种毁灭性的临床病症，影响着全球数百万人。SCI 主要影响年轻男性群体。其特征是一系列神经功能障碍，可能导致永久性残疾。本章介绍了一种改良的 SCI 技术，即 SCI 挫伤模型。该模型广泛用于研究 SCI 的病理学和测试潜在的治疗方法。通过使用压缩装置进行实验性挫伤，可通过定义特定的损伤参数来创建可重复的损伤动物模型。本文详细介绍了一种利用立体定位框架和撞击器产生可重复损伤的方法。
