大鼠严重脊髓损伤的挫伤模型
A contusion model of severe spinal cord injury in rats.
作者信息
Krishna Vibhor, Andrews Hampton, Jin Xing, Yu Jin, Varma Abhay, Wen Xuejun, Kindy Mark
机构信息
Department of Neuroscience, Division of Neurosurgery, Medical University of South Carolina, USA.
出版信息
J Vis Exp. 2013 Aug 17(78):50111. doi: 10.3791/50111.
Abstract
The translational potential of novel treatments should be investigated in severe spinal cord injury (SCI) contusion models. A detailed methodology is described to obtain a consistent model of severe SCI. Use of a stereotactic frame and computer controlled impactor allows for creation of reproducible injury. Hypothermia and urinary tract infection pose significant challenges in the post-operative period. Careful monitoring of animals with daily weight recording and bladder expression allows for early detection of post-operative complications. The functional results of this contusion model are equivalent to transection models. The contusion model can be utilized to evaluate the efficacy of both neuroprotective and neuroregenerative approaches.
摘要
新型治疗方法的转化潜力应在严重脊髓损伤（SCI）挫伤模型中进行研究。本文描述了一种详细的方法，以获得一致的严重SCI模型。使用立体定向框架和计算机控制的撞击器可产生可重复的损伤。低温和尿路感染在术后阶段构成重大挑战。通过每日记录体重和挤压膀胱对动物进行仔细监测，可早期发现术后并发症。该挫伤模型的功能结果与横断模型相当。该挫伤模型可用于评估神经保护和神经再生方法的疗效。
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本文引用的文献
1
A series of systematic reviews on the treatment of acute spinal cord injury: a foundation for best medical practice.一系列关于急性脊髓损伤治疗的系统评价：最佳医疗实践的基础。
J Neurotrauma. 2011 Aug;28(8):1329-33. doi: 10.1089/neu.2011.1955. Epub 2011 Jul 13.
2
Implantation of polymer scaffolds seeded with neural stem cells in a canine spinal cord injury model.将神经干细胞种植于聚合物支架中植入犬脊髓损伤模型。
Cytotherapy. 2010 Oct;12(6):841-5. doi: 10.3109/14653249.2010.501784.
3
A grading system to evaluate objectively the strength of pre-clinical data of acute neuroprotective therapies for clinical translation in spinal cord injury.一种用于客观评估急性神经保护疗法在脊髓损伤临床转化中临床前数据强度的分级系统。
J Neurotrauma. 2011 Aug;28(8):1525-43. doi: 10.1089/neu.2010.1296. Epub 2010 Oct 18.
4
Translational research in spinal cord injury: a survey of opinion from the SCI community.脊髓损伤转化研究：脊髓损伤领域观点调查。
J Neurotrauma. 2010 Jan;27(1):21-33. doi: 10.1089/neu.2009.1048.
5
Impact speed does not determine severity of spinal cord injury in mice with fixed impact displacement.固定撞击位移的小鼠中，撞击速度并不决定脊髓损伤的严重程度。
J Neurotrauma. 2009 Aug;26(8):1395-404. doi: 10.1089/neu.2008-0728.
6
Spinal cord injury: plasticity, regeneration and the challenge of translational drug development.脊髓损伤：可塑性、再生及转化药物开发面临的挑战
Trends Neurosci. 2009 Jan;32(1):41-7. doi: 10.1016/j.tins.2008.09.008. Epub 2008 Oct 30.
7
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J Neurotrauma. 2008 Apr;25(4):334-49. doi: 10.1089/neu.2007.0289.
8
The extent of myelin pathology differs following contusion and transection spinal cord injury.挫伤性和横断性脊髓损伤后髓鞘病理改变的程度有所不同。
J Neurotrauma. 2007 Oct;24(10):1631-46. doi: 10.1089/neu.2007.0302.
9
Curiosity and cure: translational research strategies for neural repair-mediated rehabilitation.好奇心与治愈：神经修复介导康复的转化研究策略
Dev Neurobiol. 2007 Aug;67(9):1133-47. doi: 10.1002/dneu.20514.
10
Review of treatment trials in human spinal cord injury: issues, difficulties, and recommendations.人类脊髓损伤治疗试验综述：问题、困难与建议
Neurosurgery. 2006 Nov;59(5):957-82; discussion 982-7. doi: 10.1227/01.NEU.0000245591.16087.89.
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