Krishna Vibhor, Andrews Hampton, Jin Xing, Yu Jin, Varma Abhay, Wen Xuejun, Kindy Mark
Department of Neuroscience, Division of Neurosurgery, Medical University of South Carolina, USA.
J Vis Exp. 2013 Aug 17(78):50111. doi: 10.3791/50111.
The translational potential of novel treatments should be investigated in severe spinal cord injury (SCI) contusion models. A detailed methodology is described to obtain a consistent model of severe SCI. Use of a stereotactic frame and computer controlled impactor allows for creation of reproducible injury. Hypothermia and urinary tract infection pose significant challenges in the post-operative period. Careful monitoring of animals with daily weight recording and bladder expression allows for early detection of post-operative complications. The functional results of this contusion model are equivalent to transection models. The contusion model can be utilized to evaluate the efficacy of both neuroprotective and neuroregenerative approaches.
新型治疗方法的转化潜力应在严重脊髓损伤（SCI）挫伤模型中进行研究。本文描述了一种详细的方法，以获得一致的严重SCI模型。使用立体定向框架和计算机控制的撞击器可产生可重复的损伤。低温和尿路感染在术后阶段构成重大挑战。通过每日记录体重和挤压膀胱对动物进行仔细监测，可早期发现术后并发症。该挫伤模型的功能结果与横断模型相当。该挫伤模型可用于评估神经保护和神经再生方法的疗效。
