[pC194质粒对苏云金芽孢杆菌噬菌体发育的影响]
[Effect of the pC194 plasmid on the development of Bacillus thuringiensis phages].
作者信息
Lugovskaia E V, Azizbekian R R, Stepanov A I
出版信息
Mol Gen Mikrobiol Virusol. 1985 Apr(4):22-5.
PMID:3842748
Abstract
Bacillus thuringiensis var. galleriae strains were transformed by plasmid pC194, coding for chloramphenicol resistance (CmR). Efficiency of plating and the yields of bacteriophages Tg13 and Tg27 maturating in CmR transformant cells were decreased for 2-3 orders as compared with the ones in parental strains. The CmR transformants are characterized by the increased level of spontaneous induction of bacteriophage Tg22.
摘要
用编码氯霉素抗性（CmR）的质粒pC194转化苏云金芽孢杆菌蜡螟变种菌株。与亲代菌株相比，在CmR转化细胞中平板接种效率以及噬菌体Tg13和Tg27的成熟产量降低了2 - 3个数量级。CmR转化体的特征是噬菌体Tg22的自发诱导水平增加。
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