Laboratory of Physiopathology, Molecular Genetics & Biotechnology, Faculty of Sciences Ain Chock, Health and Biotechnology Research Center, Hassan II University of Casablanca, Maarif B.P 5366, Casablanca 20000, Morocco.
Laboratory of Physiopathology, Molecular Genetics & Biotechnology, Faculty of Sciences Ain Chock, Health and Biotechnology Research Center, Hassan II University of Casablanca, Maarif B.P 5366, Casablanca 20000, Morocco; Laboratory of Medical Analysis, Andrology, LABOMAC, Casablanca, Morocco.
Res Vet Sci. 2024 Apr;170:105200. doi: 10.1016/j.rvsc.2024.105200. Epub 2024 Feb 26.
Artificial insemination is a widely adopted method in livestock production for various reasons such as health security and genetic improvement. Although sperm motility is of paramount importance in this technique as it directly influences the sperm's ability to fertilize the oocyte. In previous research on human sperm, we observed that in vitro supplementation with Origanum Vulgare essential oil significantly improved sperm motility and antioxidant activities, all without negatively affecting the integrity of their DNA. Based on these promising results, we considered it crucial to explore the potential effects of supplementation with this essential oil on sperm of other species. In this study, we studied the effects of oregano essential oil supplementation on sperm motility of (bulls = 15) (dogs = 15) and (rabbits = 9) and the changes that in vitro incubation with this oil could induce on sub-motile sperm populations of different species. The results of the study showed that in vitro oregano essential oil supplementation had a significant impact on sperm motility in the three species studied. This improvement in sperm motility was accompanied by an increase in the proportion of subpopulations with high velocity and progressivity: an increase of (2.16%, 10% and 4.84%) for subpopulation 1, (6.50%, 5.5% and 3.17%) for subpopulation 4 in bulls, dogs and rabbits respectively. While the subpopulations representing low motile and non-progressive sperm have decreased. These results suggest that the use of oregano essential oil can be a beneficial approach to improve sperm motility in different species, which can have important implications for the success of artificial insemination.
人工授精是一种广泛应用于畜牧业的方法，其原因有很多，例如健康安全和遗传改良。虽然精子活力在这项技术中至关重要，因为它直接影响精子受精卵子的能力。在我们之前对人类精子的研究中，我们观察到，体外补充牛至精油显著提高了精子的活力和抗氧化活性，同时又不会对其 DNA 的完整性产生负面影响。基于这些有希望的结果，我们认为探索这种精油对其他物种精子的潜在影响至关重要。在这项研究中，我们研究了牛至精油补充对（公牛=15）（狗=15）和（兔子=9）精子活力的影响，以及体外孵育这种油可能对不同物种的亚活力精子群体引起的变化。研究结果表明，体外牛至精油补充对三种研究物种的精子活力有显著影响。这种精子活力的提高伴随着具有高速度和渐进性的亚群比例的增加：在公牛、狗和兔子中，亚群 1 的比例分别增加了（2.16%、10%和 4.84%），亚群 4 的比例分别增加了（6.50%、5.5%和 3.17%）。而代表低活力和非渐进性精子的亚群减少了。这些结果表明，使用牛至精油可以是一种有益的方法来提高不同物种的精子活力，这对人工授精的成功具有重要意义。
