Caffin J P, Poutrel B, Rainard P
J Dairy Sci. 1985 May;68(5):1087-94. doi: 10.3168/jds.S0022-0302(85)80933-4.
Bovine alpha-lactalbumin and beta-lactoglobulin concentrations were determined by radial immunodiffusion in 354 milk samples from uninfected and 98 samples from infected quarters from 42 Holstein-Friesian cows taken at 30, 150, and 270 days of lactation. alpha-Lactalbumin and beta-lactoglobulin concentrations were not affected by quarter location. The alpha-lactalbumin decreased at the end of lactation and in samples collected beyond second lactation. The beta-lactoglobulin concentration increased with stage of lactation. There was a positive correlation between alpha-lactalbumin and beta lactoglobulin (r = .12). Milk from uninfected quarters had mean alpha-lactalbumin and beta-lactoglobulin concentrations of 1.47 and 4.6 mg/ml, respectively. Milk from quarters infected by major pathogens or Corynebacterium bovis had less alpha-lactalbumin. Milk from quarters infected by minor pathogens had less beta-lactoglobulin. There was a negative correlation between alpha-lactalbumin concentration and somatic cell count (r = .31), which was amplified by infection status of quarters. No correlation was noted between somatic cell count and beta-lactoglobulin concentration when considered over the whole sampling period, but the correlation became negative in quarters infected by major pathogens.
采用放射免疫扩散法测定了42头荷斯坦 - 弗里生奶牛在泌乳30天、150天和270天时，354份未感染乳腺的牛奶样本以及98份感染乳腺的牛奶样本中牛α - 乳白蛋白和β - 乳球蛋白的浓度。α - 乳白蛋白和β - 乳球蛋白的浓度不受乳腺位置的影响。α - 乳白蛋白在泌乳末期以及第二次泌乳后采集的样本中含量下降。β - 乳球蛋白浓度随泌乳阶段增加。α - 乳白蛋白和β - 乳球蛋白之间存在正相关（r = 0.12）。未感染乳腺的牛奶中α - 乳白蛋白和β - 乳球蛋白的平均浓度分别为1.47mg/ml和4.6mg/ml。受主要病原体或牛棒状杆菌感染的乳腺所产牛奶中α - 乳白蛋白含量较低。受次要病原体感染的乳腺所产牛奶中β - 乳球蛋白含量较低。α - 乳白蛋白浓度与体细胞计数之间存在负相关（r = 0.31），且这种负相关因乳腺的感染状态而增强。在整个采样期间，体细胞计数与β - 乳球蛋白浓度之间未发现相关性，但在受主要病原体感染的乳腺中，这种相关性变为负相关。
