2 型糖尿病中细胞衰老分子的表达：CDKN2A、CDKN2B 和 lncRNA ANRIL。
Cellular senescence molecules expression in type 2 diabetes mellitus: CDKN2A, CDKN2B, and lncRNA ANRIL.
机构信息
Department of Medical Genetics, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Endocrinology and Metabolism Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
出版信息
Gene. 2024 Jun 15;911:148319. doi: 10.1016/j.gene.2024.148319. Epub 2024 Feb 28.
AIMS
Cellular senescence in type 2 diabetes mellitus (T2DM) has received widespread attention. However, the cellular senescence molecules involved in T2DM are unclear. Furthermore, there are no consistent biomarkers for cellular senescence in T2DM. Therefore, this study aimed to identify cellular senescence molecules in T2DM and investigate their expression in peripheral blood mononuclear cells of individuals with T2DM.
METHODS
Patients with T2DM (n = 40) and healthy controls (n = 40) were enrolled. We used different databases to identify cellular senescence molecules in T2DM and confirmed the obtained genes and lncRNA using real-time PCR.
RESULTS
Bioinformatics analysis indicated that CDKN2A and CDKN2B genes, and long noncoding RNA ANRIL are the most effective cellular senescence molecules in T2DM. Furthermore, CDKN2A and ANRIL expression decreased in individuals with T2DM.
CONCLUSIONS
Cellular senescence may have a protective effect against T2DM. In addition, the cellular senescence molecules CDKN2A and ANRIL may be potential biomarkers of cellular senescence in T2DM.
目的
2 型糖尿病（T2DM）中的细胞衰老受到广泛关注。然而，涉及 T2DM 的细胞衰老分子尚不清楚。此外，T2DM 中没有一致的细胞衰老生物标志物。因此，本研究旨在鉴定 T2DM 中的细胞衰老分子，并研究其在 T2DM 个体外周血单个核细胞中的表达。
方法
纳入 40 例 T2DM 患者和 40 例健康对照者。我们使用不同的数据库来鉴定 T2DM 中的细胞衰老分子，并使用实时 PCR 来验证获得的基因和 lncRNA。
结果
生物信息学分析表明，CDKN2A 和 CDKN2B 基因以及长链非编码 RNA ANRIL 是 T2DM 中最有效的细胞衰老分子。此外，T2DM 个体中 CDKN2A 和 ANRIL 的表达降低。
结论
细胞衰老可能对 T2DM 具有保护作用。此外，细胞衰老分子 CDKN2A 和 ANRIL 可能是 T2DM 中细胞衰老的潜在生物标志物。
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