Li Xinyi, Yang Aige, Liu Xiao, Zhang Rui, Zhou Huimin, Xu Shunjiang
Department of Emergency, The First Hospital of Hebei Medical University, Shijiazhuang, 050031, China.
Department of Endocrinology and Metabolism, The First Hospital of Hebei Medical University, Shijiazhuang, 050031, China.
Diabetol Metab Syndr. 2025 Mar 28;17(1):108. doi: 10.1186/s13098-025-01670-3.
Type 2 diabetes mellitus (T2DM) is closely associated with both environmental and genetic factors, involving multi-gene inheritance. This study examined the association between the polymorphic locus rs10757278 in long non-coding RNA ANRIL and T2DM.
Polymerase chain reaction (PCR) was used to detect the rs10757278 polymorphism in the ANRIL gene. RT-qPCR measured ANRIL expression levels, and logistic regression identified independent risk factors for T2DM. Furthermore, the receiver operating characteristic (ROC) curve was constructed to evaluate the clinical diagnostic value of serum ANRIL levels in diagnosing T2DM.
The rs10757278 polymorphism of the ANRIL was associated with the development of T2DM. Specifically, the G allele increases the risk of T2DM, and individuals carrying the GG genotype have a higher risk of developing the disease. Significant differences were found in low-density lipoprotein cholesterol (LDL-C), fasting plasma glucose (FPG), and glycated hemoglobin (HbA1c) among T2DM patients with different genotypes of ANRIL rs10757278. The relative FPG and HbA1c levels were relatively lower in individuals with the AA genotype and higher in those with the GG genotype. Moreover, serum ANRIL levels in the T2DM group were lower than in the control group. Body mass index (BMI), the rs10757278 locus, and serum ANRIL levels were independent risk factors for the development of T2DM. The ROC curve showed that serum ANRIL levels have significant clinical diagnostic value for the diagnosis of T2DM.
The rs10757278 polymorphism in ANRIL was strongly associated with the genetic predisposition to T2DM.
2型糖尿病（T2DM）与环境和遗传因素密切相关，涉及多基因遗传。本研究检测长链非编码RNA ANRIL中多态性位点rs10757278与T2DM之间的关联。
采用聚合酶链反应（PCR）检测ANRIL基因中的rs10757278多态性。逆转录定量聚合酶链反应（RT-qPCR）测定ANRIL表达水平，逻辑回归分析确定T2DM的独立危险因素。此外，构建受试者工作特征（ROC）曲线以评估血清ANRIL水平对T2DM的临床诊断价值。
ANRIL的rs10757278多态性与T2DM的发生有关。具体而言，G等位基因增加了T2DM的发病风险，携带GG基因型的个体患该病的风险更高。在ANRIL rs10757278不同基因型的T2DM患者中，低密度脂蛋白胆固醇（LDL-C）、空腹血糖（FPG）和糖化血红蛋白（HbA1c）存在显著差异。AA基因型个体的相对FPG和HbA1c水平较低，GG基因型个体的相对FPG和HbA1c水平较高。此外，T2DM组的血清ANRIL水平低于对照组。体重指数（BMI）、rs10757278位点和血清ANRIL水平是T2DM发生的独立危险因素。ROC曲线显示，血清ANRIL水平对T2DM的诊断具有显著的临床诊断价值。
ANRIL中的rs10757278多态性与T2DM的遗传易感性密切相关。
