Dermatology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
Arch Dermatol Res. 2024 Mar 2;316(3):97. doi: 10.1007/s00403-024-02838-z.
Programmed cell death protein-1 (PD-1) is an immune checkpoint protein, PD-1 interaction with PD ligand-1 (PD-L1) is essential for maintaining immunological tolerance. The study aimed to study and compare the levels of PD-1 and PD-L1 in lesional and nonlesional skin of lichen planus (LP) patients and compare these levels to normal healthy controls to assess their role in the pathogenesis of LP. This case-control study involved 30 patients with LP and 30 healthy age-and sex-matched controls. After clinical assessment of the severity by LP severity index score (LPSI), skin biopsies were taken from lesional and nonlesional skin of LP patients and from normal skin in healthy controls for assessment of the tissue levels of PD-1 and PD-L1 by ELISA. The tissue levels of both PD-1 and PD-L1 were significantly higher in healthy controls than in both lesional and nonlesional skin of LP patients (P < 0.001). Also, significantly higher PD-l and PD-L1 levels in nonlesional skin than in lesional skin of LP patients were reported (P < 0.001). No significant correlations were found between lesional and nonlesional PD-1, PD-L1 levels, or LPSI score. Based on the fact that PD-1/PD-L1 interaction is important to maintain tolerance and protection against autoimmune diseases, in addition to our study results that revealed lower levels of PD-1/PD-L1 in LP skin than in healthy skin, we can conclude that PD-1/PDL-1 may be incriminated in the pathogenesis of LP. ClinicalTrials.govID: NCT04892381.
程序性细胞死亡蛋白-1（PD-1）是一种免疫检查点蛋白，PD-1 与 PD 配体-1（PD-L1）的相互作用对于维持免疫耐受至关重要。本研究旨在研究和比较扁平苔藓（LP）患者皮损和非皮损皮肤中 PD-1 和 PD-L1 的水平，并将这些水平与正常健康对照进行比较，以评估它们在 LP 发病机制中的作用。这项病例对照研究纳入了 30 名 LP 患者和 30 名年龄和性别匹配的健康对照者。通过 LP 严重程度指数评分（LPSI）对严重程度进行临床评估后，从 LP 患者的皮损和非皮损皮肤以及健康对照者的正常皮肤中采集皮肤活检组织，通过 ELISA 评估 PD-1 和 PD-L1 的组织水平。与 LP 患者的皮损和非皮损皮肤相比，健康对照者的 PD-1 和 PD-L1 组织水平均显著更高（P<0.001）。此外，LP 患者的非皮损皮肤的 PD-l 和 PD-L1 水平也显著高于皮损皮肤（P<0.001）。LP 患者皮损和非皮损 PD-1、PD-L1 水平与 LPSI 评分之间无显著相关性。鉴于 PD-1/PD-L1 相互作用对于维持耐受和预防自身免疫性疾病非常重要，再加上我们的研究结果表明 LP 皮肤中的 PD-1/PD-L1 水平低于健康皮肤，我们可以得出结论，PD-1/PDL-1 可能与 LP 的发病机制有关。ClinicalTrials.govID：NCT04892381。
