Wang Yuhong, Wei Minghui, Wang Yuanyuan, Liu Yuan, Wang Xinwen
Department of Oral Medicine, School of Stomatology, The Fourth Military Medical University (FMMU), Xi'an, China.
Shaanxi Clinical Research Center for Oral Diseases, National Clinical Research Center for Oral Diseases, State Key Laboratory of Military Stomatology, Xi'an, China.
J Oral Pathol Med. 2023 Jan;52(1):72-80. doi: 10.1111/jop.13383. Epub 2022 Nov 17.
The chemokines play a crucial role in the recruitment of lymphocytes in oral lichen planus, and the activated epithelial cells are the main producers of the chemokines. However, the signals provoking chemokine secretion still remain to be elucidated.
The global expression profile of chemokines in oral epithelial cell line induced by IFNγ was determined by microarray analysis. The gene and protein expression was validated in primary culture of oral epithelial cells, and the effects of IFNγ on regulating chemokine production were compared with that of TNFα and IL2. Moreover, the capability of primary culture of oral epithelial cells to attract peripheral lymphocytes in response to IFNγ was investigated in oral lichen planus patients, and the cell phenotype of the recruited lymphocytes was analyzed using flow cytometry.
IFNγ triggered the expression of multiple chemokines in the oral epithelial cells. The expression pattern of the chemokines closely resembled that in the epithelial cell layer of oral lichen planus lesions. Compared with IL2 and TNFα, IFNγ demonstrated a distinct maximal effect on the chemokines secretion in primary culture of oral epithelial cells. The migration of peripheral lymphocytes toward the culture supernatant of IFNγ-treated primary culture of oral epithelial cells was significantly enhanced in the oral lichen planus group compared to that in the healthy control group.
IFNγ plays an important role in the chemokine secretion and epidermotropic migration of lymphocytes in oral lichen planus.
趋化因子在口腔扁平苔藓淋巴细胞募集中起关键作用，活化的上皮细胞是趋化因子的主要产生者。然而，引发趋化因子分泌的信号仍有待阐明。
通过微阵列分析确定IFNγ诱导的口腔上皮细胞系中趋化因子的整体表达谱。在口腔上皮细胞原代培养中验证基因和蛋白表达，并将IFNγ对趋化因子产生的调节作用与TNFα和IL2进行比较。此外，在口腔扁平苔藓患者中研究口腔上皮细胞原代培养物响应IFNγ吸引外周淋巴细胞的能力，并使用流式细胞术分析募集淋巴细胞的细胞表型。
IFNγ触发口腔上皮细胞中多种趋化因子的表达。趋化因子的表达模式与口腔扁平苔藓病变上皮细胞层中的表达模式非常相似。与IL2和TNFα相比，IFNγ对口腔上皮细胞原代培养中趋化因子分泌表现出明显的最大效应。与健康对照组相比，口腔扁平苔藓组中外周淋巴细胞向IFNγ处理的口腔上皮细胞原代培养物上清液的迁移明显增强。
IFNγ在口腔扁平苔藓趋化因子分泌和淋巴细胞向表皮迁移中起重要作用。
